Topical treatment with nerve growth factor in an animal model of herpetic keratitis

Alessandro Lambiase, Marco Coassin, Nicola Costa, Paolo Lauretti, Alessandra Micera, Emiliano Ghinelli, Luigi Aloe, Paolo Rama, Stefano Bonini

Research output: Contribution to journalArticlepeer-review

Abstract

Background: In vitro and in vivo studies demonstrated the antiviral efficacy of nerve growth factor (NGF) and its cyto-protective effect in herpes simplex virus (HSV)-infected cells. The aims of this study were to evaluate the role of endogenous NGF in HSV corneal infection, and the effects of topical NGF treatment on herpetic keratitis. Methods: Herpetic keratitis was induced in 40 rabbits with the HSV-1 McKrae strain. Animals were divided into four groups, and treated with topical neutralizing anti-NGF antibodies, NGF, acyclovir or balanced salt solution (BSS) respectively. The clinical course of HSV keratitis was evaluated and scored by slit-lamp examination. In addition, biochemical (immunohistochemistry for glycoprotein D) and molecular (nested PCR for glycoprotein D) analyses were carried out to estimate viral replication. Results: Treatment with anti-NGF antibodies induced a more severe keratitis associated with increased biochemical and molecular markers of active viral replication. Two animals in this group developed lethal HSV encephalitis. Conversely, topical treatment with NGF induced a significant amelioration of clinical and laboratory parameters when compared to the BSS treated group (control). No significant differences were observed between NGF- and acyclovir-treated groups. Conclusions: This study demonstrated the crucial role of endogenous NGF in herpetic keratitis. The comparable effects of NGF and acyclovir confirm the antiviral activity of NGF, and indicate a potential use of topical NGF in herpetic keratitis.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume246
Issue number1
DOIs
Publication statusPublished - Jan 2008

Keywords

  • Antiviral therapy
  • Herpes simplex virus
  • Herpetic keratitis
  • Nerve growth factor

ASJC Scopus subject areas

  • Ophthalmology

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