Topology of the mitochondrial cAMP-dependent protein kinase and its substrates

Anna Maria Sardanelli, Zuzana Technikova-Dobrova, Francesco Speranza, Antonio Mazzocca, Salvatore Scacco, Sergio Papa

Research output: Contribution to journalArticle

Abstract

In intact bovine heart mitochondria, cAMP-dependent phosphorylation of 42, 29, 18 and 6.5 kDa proteins was inhibited by carboxyatractyloside. This shows that both mitochondrial cAMP-dependent protein kinase (mtPKA) and its protein substrates are localized at the matrix side of the inner mitochondrial membrane. Proteins of 42, 29, 18, and 6.5 kDa were also bound at the outer surface of mitochondria where they were phosphorylated by the added purified catalytic subunit of PKA. In the cytosol from bovine heart proteins of the above molecular weights mere phosphorylated by the cytosolic PKA.

Original languageEnglish
Pages (from-to)276-278
Number of pages3
JournalFEBS Letters
Volume396
Issue number2-3
DOIs
Publication statusPublished - Nov 4 1996

Fingerprint

Cyclic AMP-Dependent Protein Kinases
Topology
Mitochondria
Substrates
Proteins
Heart Mitochondria
Phosphorylation
Mitochondrial Membranes
Cytosol
Catalytic Domain
Molecular Weight
Molecular weight
Membranes

Keywords

  • Cyclic AMP
  • Cyclic AMP-dependent protein kinase
  • Mitochondrion
  • Protein kinase
  • Protein phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sardanelli, A. M., Technikova-Dobrova, Z., Speranza, F., Mazzocca, A., Scacco, S., & Papa, S. (1996). Topology of the mitochondrial cAMP-dependent protein kinase and its substrates. FEBS Letters, 396(2-3), 276-278. https://doi.org/10.1016/0014-5793(96)01112-X

Topology of the mitochondrial cAMP-dependent protein kinase and its substrates. / Sardanelli, Anna Maria; Technikova-Dobrova, Zuzana; Speranza, Francesco; Mazzocca, Antonio; Scacco, Salvatore; Papa, Sergio.

In: FEBS Letters, Vol. 396, No. 2-3, 04.11.1996, p. 276-278.

Research output: Contribution to journalArticle

Sardanelli, AM, Technikova-Dobrova, Z, Speranza, F, Mazzocca, A, Scacco, S & Papa, S 1996, 'Topology of the mitochondrial cAMP-dependent protein kinase and its substrates', FEBS Letters, vol. 396, no. 2-3, pp. 276-278. https://doi.org/10.1016/0014-5793(96)01112-X
Sardanelli AM, Technikova-Dobrova Z, Speranza F, Mazzocca A, Scacco S, Papa S. Topology of the mitochondrial cAMP-dependent protein kinase and its substrates. FEBS Letters. 1996 Nov 4;396(2-3):276-278. https://doi.org/10.1016/0014-5793(96)01112-X
Sardanelli, Anna Maria ; Technikova-Dobrova, Zuzana ; Speranza, Francesco ; Mazzocca, Antonio ; Scacco, Salvatore ; Papa, Sergio. / Topology of the mitochondrial cAMP-dependent protein kinase and its substrates. In: FEBS Letters. 1996 ; Vol. 396, No. 2-3. pp. 276-278.
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