Abstract
The molecular mechanisms necessary for remyelination by oligodendrocytes remain unexplored. We previously characterized a myelin basic protein promoter-lacZ (MBP-lacZ) transgene whose expression is regulated uniquely during development, and also in pathological situations, suggesting that it may be a useful reporter of molecular mechanisms during remyelination. As a first step toward creating a transgenic mouse model of remyelination, we cultured oligodendrocytes from these transgenic mice and showed that expression of MBP-lacZ appeared in parallel with a marker of oligodendrocyte maturation, galactocerebroside (GC). In addition, basic fibroblast growth factor blocked the expression of both MBP-lacZ and GC in these cells. Therefore, expression of MBP-lacZ reflects not only the developmental stage of oligodendrocytes, but also extrinsic influences on oligodendrocytes. These data suggest that MBP-lacZ may be a useful marker in transgenic mouse models of remyelination.
Original language | English |
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Pages (from-to) | 80-83 |
Number of pages | 4 |
Journal | Multiple Sclerosis Journal |
Volume | 3 |
Issue number | 2 |
Publication status | Published - Apr 1997 |
Keywords
- β-galactosidase
- Fibroblast growth factor
- Myelin basic protein promoter
- Oligodendrocytes
- Platelet derived growth factor
- Remyelination
ASJC Scopus subject areas
- Clinical Neurology