Toward harmonized phenotyping of human myeloid-derived suppressor cells by flow cytometry: results from an interim study

Susanna Mandruzzato, Sven Brandau, Cedrik M. Britten, Vincenzo Bronte, Vera Damuzzo, Cécile Gouttefangeas, Dominik Maurer, Christian Ottensmeier, Sjoerd H. van der Burg, Marij J P Welters, Steffen Walter

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

There is an increasing interest for monitoring circulating myeloid-derived suppressor cells (MDSCs) in cancer patients, but there are also divergences in their phenotypic definition. To overcome this obstacle, the Cancer Immunoguiding Program under the umbrella of the Association of Cancer Immunotherapy is coordinating a proficiency panel program that aims at harmonizing MDSC phenotyping. After a consultation period, a two-stage approach was designed to harmonize MDSC phenotype. In the first step, an international consortium of 23 laboratories immunophenotyped 10 putative MDSC subsets on pretested, peripheral blood mononuclear cells of healthy donors to assess the level of concordance and define robust marker combinations for the identification of circulating MDSCs. At this stage, no mandatory requirements to standardize reagents or protocols were introduced. Data analysis revealed a small intra-laboratory, but very high inter-laboratory variance for all MDSC subsets, especially for the granulocytic subsets. In particular, the use of a dead-cell marker altered significantly the reported percentage of granulocytic MDSCs, confirming that these cells are especially sensitive to cryopreservation and/or thawing. Importantly, the gating strategy was heterogeneous and associated with high inter-center variance. Overall, our results document the high variability in MDSC phenotyping in the multicenter setting if no harmonization/standardization measures are applied. Although the observed variability depended on a number of identified parameters, the main parameter associated with variation was the gating strategy. Based on these findings, we propose further efforts to harmonize marker combinations and gating parameters to identify strategies for a robust enumeration of MDSC subsets.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalCancer Immunology, Immunotherapy
Volume65
Issue number2
DOIs
Publication statusPublished - Feb 1 2016

Fingerprint

Flow Cytometry
Myeloid-Derived Suppressor Cells
Neoplasms
Cryopreservation
Immunotherapy
Blood Cells
Referral and Consultation
Tissue Donors
Phenotype

Keywords

  • Flow cytometry
  • Myeloid-derived suppressor cells
  • Phenotyping
  • Proficiency panel

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Immunology
  • Immunology and Allergy

Cite this

Toward harmonized phenotyping of human myeloid-derived suppressor cells by flow cytometry : results from an interim study. / Mandruzzato, Susanna; Brandau, Sven; Britten, Cedrik M.; Bronte, Vincenzo; Damuzzo, Vera; Gouttefangeas, Cécile; Maurer, Dominik; Ottensmeier, Christian; van der Burg, Sjoerd H.; Welters, Marij J P; Walter, Steffen.

In: Cancer Immunology, Immunotherapy, Vol. 65, No. 2, 01.02.2016, p. 161-169.

Research output: Contribution to journalArticle

Mandruzzato, S, Brandau, S, Britten, CM, Bronte, V, Damuzzo, V, Gouttefangeas, C, Maurer, D, Ottensmeier, C, van der Burg, SH, Welters, MJP & Walter, S 2016, 'Toward harmonized phenotyping of human myeloid-derived suppressor cells by flow cytometry: results from an interim study', Cancer Immunology, Immunotherapy, vol. 65, no. 2, pp. 161-169. https://doi.org/10.1007/s00262-015-1782-5
Mandruzzato, Susanna ; Brandau, Sven ; Britten, Cedrik M. ; Bronte, Vincenzo ; Damuzzo, Vera ; Gouttefangeas, Cécile ; Maurer, Dominik ; Ottensmeier, Christian ; van der Burg, Sjoerd H. ; Welters, Marij J P ; Walter, Steffen. / Toward harmonized phenotyping of human myeloid-derived suppressor cells by flow cytometry : results from an interim study. In: Cancer Immunology, Immunotherapy. 2016 ; Vol. 65, No. 2. pp. 161-169.
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