Toward multiprotein nanoarrays using nanografting and DNA directed immobilization of proteins

Fouzia Bano, Ljiljana Fruk, Barbara Sanavio, Maximilian Glettenberg, Loredana Casalis, Christof M. Niemeyer, Giacinto Scoles

Research output: Contribution to journalArticlepeer-review

Abstract

Atomic force microscopy nanografting was utilized to prepare DNA nanopatches of different sizes (200 × 200 to 1000 × 1000 nm 2) onto which DNA-protein conjugates can be anchored through DNA-directed immobilization. Height measurements were used to assess the binding of the proteins as well as their subsequent interaction with other components, such as antibodies. The results indicate that nanografted patch arrays are well suited for application in biosensing and could enable the fabrication of multifeature protein nanoarrays.

Original languageEnglish
Pages (from-to)2614-2618
Number of pages5
JournalNano Letters
Volume9
Issue number7
DOIs
Publication statusPublished - Jul 8 2009

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Mechanical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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