Toward the standardization of CD34+ haematopoietic progenitor cell estimation in human umbilical cord blood

G. D'Arena, N. Dello Iacono, P. Musto, L. Di Mauro, G. Perla, N. Cascavilla, M. Carotenuto

Research output: Contribution to journalArticlepeer-review

Abstract

The most widely used procedure for enumerating CD34+ haematopoietic progenitors is flow cytometry. Estimation of these cells in human umbilical cord blood (HUCB), which is now more and more frequently used for transplantation, has the disadvantage of nucleated red cell contamination resisting to the ammonium chloride lysis. Since erythroblasts circulating in HUCB are CD45 negative, we performed a procedure for the measurement of CD34+ progenitor cells on whole HUCB based on a double staining with CD34- phycoerythrin (PE) and CD45-fluorescein isothiocyanate (FITC) directly conjugated antibodies. Estimating CD34+ cells gated on CD45+ cells (leucocytes), we obtained a greater mean number of cells (0.35% ± 0.18) with respect to an estimation on the total cells (without CD45-cell exclusion) (0.27% ± 0.18). Such a difference was statistically significant (p <0.005). In addition, a linear regression analysis showed a strong correlation (r 0.8; p

Original languageEnglish
Pages (from-to)98-102
Number of pages5
JournalTrasfusione del Sangue
Volume43
Issue number2
Publication statusPublished - 1998

Keywords

  • CD34
  • CD45
  • Flow cytometry
  • Human umbilical cord blood

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy

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