Towards ideal magnetofluorescent nanoparticles for bimodal detection of breast-cancer cells

Fabio Corsi, Clara De Palma, Miriam Colombo, Raffaele Allevi, Manuela Nebuloni, Silvia Ronchi, Giuseppina Rizzi, Antonella Tosoni, Emilio Trabucchi, Emilio Clementi, Davide Prosperi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

An increasing number of novel molecular markers based on nanomaterials for tumor diagnostics have been developed in recent years. Many efforts have focused on the achievement of site-targeted bioconjugated nanoparticles. In contrast, the mechanisms of toxicity, endocytosis, and degradation pathways are still poorly understood, despite their primary importance for clinical translation. In this study, three different model nanoscale magnetofluorescent par icle sys ems (MFNs) are designed and fabrica ed. These nanopar icles are evaluated in terms of size, morphology, zeta potential, fluorescence efficiency, capability of enhancing T2 relaxivity of water protons, and stability. Accordingly, two are developed and the mechanism of internalization, the intracellular fate, and the toxicity in MCF-7 adenocarcinoma cells are studied. Besides the well-documented size effect, the anionic charge seems to be a crucial factor for particle internalization, as MFN penetration through the cell membrane could be modulated by surface charge. Ultrastructural analysis of transmission electron micrographs combined with evidence from confocal microscopy reveals that MFNs are internalized by clathrin-mediated endocytosis and macropinocytosis. Moreover, MFNs are found in EEA1-positive endosomes and in lysosomes, indicating that they follow a physiological pathway of endocytosis. Magnetorelaxometric analysis demonstrates that MFNs enable the detection of 5 × 105 cells mL-1 after treatment with particle dosages as low as 30 μg mL-1. Hence, MFNs appear to be a valuable and safe bimodal contrast agent that can be developed for the noninvasive diagnosis of breast cancer.

Original languageEnglish
Pages (from-to)2555-2564
Number of pages10
JournalSmall
Volume5
Issue number22
DOIs
Publication statusPublished - Nov 16 2009

Fingerprint

Endocytosis
Nanoparticles
Toxicity
Cells
Breast Neoplasms
Clathrin
Confocal microscopy
Zeta potential
Cell membranes
Surface charge
Nanostructured materials
Contrast Media
Protons
Tumors
Nanostructures
Endosomes
Fluorescence
MCF-7 Cells
Lysosomes
Confocal Microscopy

Keywords

  • Cells
  • Magnetic materials
  • Nanoparticles
  • Toxicity
  • Tumor diagnostics

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

Cite this

Corsi, F., De Palma, C., Colombo, M., Allevi, R., Nebuloni, M., Ronchi, S., ... Prosperi, D. (2009). Towards ideal magnetofluorescent nanoparticles for bimodal detection of breast-cancer cells. Small, 5(22), 2555-2564. https://doi.org/10.1002/smll.200900881

Towards ideal magnetofluorescent nanoparticles for bimodal detection of breast-cancer cells. / Corsi, Fabio; De Palma, Clara; Colombo, Miriam; Allevi, Raffaele; Nebuloni, Manuela; Ronchi, Silvia; Rizzi, Giuseppina; Tosoni, Antonella; Trabucchi, Emilio; Clementi, Emilio; Prosperi, Davide.

In: Small, Vol. 5, No. 22, 16.11.2009, p. 2555-2564.

Research output: Contribution to journalArticle

Corsi, F, De Palma, C, Colombo, M, Allevi, R, Nebuloni, M, Ronchi, S, Rizzi, G, Tosoni, A, Trabucchi, E, Clementi, E & Prosperi, D 2009, 'Towards ideal magnetofluorescent nanoparticles for bimodal detection of breast-cancer cells', Small, vol. 5, no. 22, pp. 2555-2564. https://doi.org/10.1002/smll.200900881
Corsi F, De Palma C, Colombo M, Allevi R, Nebuloni M, Ronchi S et al. Towards ideal magnetofluorescent nanoparticles for bimodal detection of breast-cancer cells. Small. 2009 Nov 16;5(22):2555-2564. https://doi.org/10.1002/smll.200900881
Corsi, Fabio ; De Palma, Clara ; Colombo, Miriam ; Allevi, Raffaele ; Nebuloni, Manuela ; Ronchi, Silvia ; Rizzi, Giuseppina ; Tosoni, Antonella ; Trabucchi, Emilio ; Clementi, Emilio ; Prosperi, Davide. / Towards ideal magnetofluorescent nanoparticles for bimodal detection of breast-cancer cells. In: Small. 2009 ; Vol. 5, No. 22. pp. 2555-2564.
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