Towards intervertebral disc engineering: Bio-mimetics of form and function of the annulus fibrosus lamellae

Mirit Sharabi, Shir Wertheimer, Kelly R. Wade, Fabio Galbusera, Dafna Benayahu, Hans Joachim Wilke, Rami Haj-Ali

Research output: Contribution to journalArticle

Abstract

The aging western society is heavily afflicted with intervertebral disc (IVD) degeneration. Replacement or repair of the degenerated IVD with an artificial bio-mimetic construct is one of the challenges of future research due to its complex structure and unique biomechanical function. Herein, biocomposite laminates made of long collagen fibers in unidirectional (−1.3 ± 2.1°) and angle-plied ± 30° orientations (30.4 ± 6.4 and −29.8 ± 4.5), embedded in alginate hydrogel, were fabricated to mimic the form of single annulus fibrosus (AF) lamella and the circumferential AF, respectively. The mechanical behavior of the composites was measured and compared with in vitro existing data of the human native AF as well as with new data obtained from ovine and bovine specimens. The mechanical behavior was found to reproduce the full stress- strain behavior of the human AF single lamella in several regions of the AF and the Young's modulus was 28.3 ± 8.6 MPa. Moreover, the modulus of the angle-plied laminates was 16.8 ± 2.9 MPa, which is approximately 5% less than the in vitro data. The full stress-strain behavior was also compared with bovine and ovine circumferential AF samples and found to be very similar, with a difference in the modulus of 4.1% and 19.7%, respectively. Moreover, an FE model of the L3-L4 functional spinal unit (FSU) was developed and calibrated to evaluate the mechanical ability of the biocomposite to be used as an AF substitute under physiological IVD loading modes. The biocomposite demonstrated a good ability to mimic the stiffness of the native tissue under physiologic loading modes as flexion, extension, lateral bending and compression, but was too flexible under torsion. It was found that the proposed biomimetics AF design resulted in a compatible function in several mechanical levels, which holds great potential to be used as a viable AF replacement towards full IVD engineering.

Original languageEnglish
Pages (from-to)298-307
Number of pages10
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume94
DOIs
Publication statusPublished - Jun 1 2019

Fingerprint

Laminates
Hydrogel
Alginate
Biomimetics
Collagen
Hydrogels
Torsional stress
Repair
Aging of materials
Elastic moduli
Stiffness
Tissue
Fibers
Composite materials
alginic acid

Keywords

  • Annulus fibrosus
  • Biocomposite
  • Biomimetics
  • Collagen fibers
  • Laminate
  • Nonlinear mechanical behavior
  • Stress-strain

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Mechanics of Materials

Cite this

Towards intervertebral disc engineering : Bio-mimetics of form and function of the annulus fibrosus lamellae. / Sharabi, Mirit; Wertheimer, Shir; Wade, Kelly R.; Galbusera, Fabio; Benayahu, Dafna; Wilke, Hans Joachim; Haj-Ali, Rami.

In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 94, 01.06.2019, p. 298-307.

Research output: Contribution to journalArticle

Sharabi, Mirit ; Wertheimer, Shir ; Wade, Kelly R. ; Galbusera, Fabio ; Benayahu, Dafna ; Wilke, Hans Joachim ; Haj-Ali, Rami. / Towards intervertebral disc engineering : Bio-mimetics of form and function of the annulus fibrosus lamellae. In: Journal of the Mechanical Behavior of Biomedical Materials. 2019 ; Vol. 94. pp. 298-307.
@article{0a89fcfb2b594ba7b639631ef72d71ac,
title = "Towards intervertebral disc engineering: Bio-mimetics of form and function of the annulus fibrosus lamellae",
abstract = "The aging western society is heavily afflicted with intervertebral disc (IVD) degeneration. Replacement or repair of the degenerated IVD with an artificial bio-mimetic construct is one of the challenges of future research due to its complex structure and unique biomechanical function. Herein, biocomposite laminates made of long collagen fibers in unidirectional (−1.3 ± 2.1°) and angle-plied ± 30° orientations (30.4 ± 6.4 and −29.8 ± 4.5), embedded in alginate hydrogel, were fabricated to mimic the form of single annulus fibrosus (AF) lamella and the circumferential AF, respectively. The mechanical behavior of the composites was measured and compared with in vitro existing data of the human native AF as well as with new data obtained from ovine and bovine specimens. The mechanical behavior was found to reproduce the full stress- strain behavior of the human AF single lamella in several regions of the AF and the Young's modulus was 28.3 ± 8.6 MPa. Moreover, the modulus of the angle-plied laminates was 16.8 ± 2.9 MPa, which is approximately 5{\%} less than the in vitro data. The full stress-strain behavior was also compared with bovine and ovine circumferential AF samples and found to be very similar, with a difference in the modulus of 4.1{\%} and 19.7{\%}, respectively. Moreover, an FE model of the L3-L4 functional spinal unit (FSU) was developed and calibrated to evaluate the mechanical ability of the biocomposite to be used as an AF substitute under physiological IVD loading modes. The biocomposite demonstrated a good ability to mimic the stiffness of the native tissue under physiologic loading modes as flexion, extension, lateral bending and compression, but was too flexible under torsion. It was found that the proposed biomimetics AF design resulted in a compatible function in several mechanical levels, which holds great potential to be used as a viable AF replacement towards full IVD engineering.",
keywords = "Annulus fibrosus, Biocomposite, Biomimetics, Collagen fibers, Laminate, Nonlinear mechanical behavior, Stress-strain",
author = "Mirit Sharabi and Shir Wertheimer and Wade, {Kelly R.} and Fabio Galbusera and Dafna Benayahu and Wilke, {Hans Joachim} and Rami Haj-Ali",
year = "2019",
month = "6",
day = "1",
doi = "10.1016/j.jmbbm.2019.03.023",
language = "English",
volume = "94",
pages = "298--307",
journal = "Journal of the Mechanical Behavior of Biomedical Materials",
issn = "1751-6161",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Towards intervertebral disc engineering

T2 - Bio-mimetics of form and function of the annulus fibrosus lamellae

AU - Sharabi, Mirit

AU - Wertheimer, Shir

AU - Wade, Kelly R.

AU - Galbusera, Fabio

AU - Benayahu, Dafna

AU - Wilke, Hans Joachim

AU - Haj-Ali, Rami

PY - 2019/6/1

Y1 - 2019/6/1

N2 - The aging western society is heavily afflicted with intervertebral disc (IVD) degeneration. Replacement or repair of the degenerated IVD with an artificial bio-mimetic construct is one of the challenges of future research due to its complex structure and unique biomechanical function. Herein, biocomposite laminates made of long collagen fibers in unidirectional (−1.3 ± 2.1°) and angle-plied ± 30° orientations (30.4 ± 6.4 and −29.8 ± 4.5), embedded in alginate hydrogel, were fabricated to mimic the form of single annulus fibrosus (AF) lamella and the circumferential AF, respectively. The mechanical behavior of the composites was measured and compared with in vitro existing data of the human native AF as well as with new data obtained from ovine and bovine specimens. The mechanical behavior was found to reproduce the full stress- strain behavior of the human AF single lamella in several regions of the AF and the Young's modulus was 28.3 ± 8.6 MPa. Moreover, the modulus of the angle-plied laminates was 16.8 ± 2.9 MPa, which is approximately 5% less than the in vitro data. The full stress-strain behavior was also compared with bovine and ovine circumferential AF samples and found to be very similar, with a difference in the modulus of 4.1% and 19.7%, respectively. Moreover, an FE model of the L3-L4 functional spinal unit (FSU) was developed and calibrated to evaluate the mechanical ability of the biocomposite to be used as an AF substitute under physiological IVD loading modes. The biocomposite demonstrated a good ability to mimic the stiffness of the native tissue under physiologic loading modes as flexion, extension, lateral bending and compression, but was too flexible under torsion. It was found that the proposed biomimetics AF design resulted in a compatible function in several mechanical levels, which holds great potential to be used as a viable AF replacement towards full IVD engineering.

AB - The aging western society is heavily afflicted with intervertebral disc (IVD) degeneration. Replacement or repair of the degenerated IVD with an artificial bio-mimetic construct is one of the challenges of future research due to its complex structure and unique biomechanical function. Herein, biocomposite laminates made of long collagen fibers in unidirectional (−1.3 ± 2.1°) and angle-plied ± 30° orientations (30.4 ± 6.4 and −29.8 ± 4.5), embedded in alginate hydrogel, were fabricated to mimic the form of single annulus fibrosus (AF) lamella and the circumferential AF, respectively. The mechanical behavior of the composites was measured and compared with in vitro existing data of the human native AF as well as with new data obtained from ovine and bovine specimens. The mechanical behavior was found to reproduce the full stress- strain behavior of the human AF single lamella in several regions of the AF and the Young's modulus was 28.3 ± 8.6 MPa. Moreover, the modulus of the angle-plied laminates was 16.8 ± 2.9 MPa, which is approximately 5% less than the in vitro data. The full stress-strain behavior was also compared with bovine and ovine circumferential AF samples and found to be very similar, with a difference in the modulus of 4.1% and 19.7%, respectively. Moreover, an FE model of the L3-L4 functional spinal unit (FSU) was developed and calibrated to evaluate the mechanical ability of the biocomposite to be used as an AF substitute under physiological IVD loading modes. The biocomposite demonstrated a good ability to mimic the stiffness of the native tissue under physiologic loading modes as flexion, extension, lateral bending and compression, but was too flexible under torsion. It was found that the proposed biomimetics AF design resulted in a compatible function in several mechanical levels, which holds great potential to be used as a viable AF replacement towards full IVD engineering.

KW - Annulus fibrosus

KW - Biocomposite

KW - Biomimetics

KW - Collagen fibers

KW - Laminate

KW - Nonlinear mechanical behavior

KW - Stress-strain

UR - http://www.scopus.com/inward/record.url?scp=85063741419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063741419&partnerID=8YFLogxK

U2 - 10.1016/j.jmbbm.2019.03.023

DO - 10.1016/j.jmbbm.2019.03.023

M3 - Article

C2 - 30951990

AN - SCOPUS:85063741419

VL - 94

SP - 298

EP - 307

JO - Journal of the Mechanical Behavior of Biomedical Materials

JF - Journal of the Mechanical Behavior of Biomedical Materials

SN - 1751-6161

ER -