Toxicity of cyanoacrylates in vitro using extract dilution assay on cell cultures

Gabriela Ciapetti, Susanna Stea, Elisabetta Cenni, Alessandra Sudanese, Daniela Marraro, Aldo Toni, Arturo Pizzoferrato

Research output: Contribution to journalArticlepeer-review


Comparative cytotoxicity testing of four cyanoacrylate adhesives suggested for orthopaedic applications was performed. These substances were placed in complete culture medium with serum and the resulting extraction fluids were tested on L 929 cells and human lymphocytes. Testing procedures include cell morphology assessment using light microscopy and vital dyes, cell counting using a computer-assisted image analysis system, cell growth measurement using total protein content assay and cell viability assessment using the MTT method. Quantitation of the toxicity of the degradation products released by cyanoacrylates in the extracts was achieved and differences in the cytopathic effect related to the chemical composition of the cyanoacrylates were found. A toxicity rating of the assayed cyanoacrylate adhesives was obtained as follows (in order of increasing toxicity): BCA <xCA <ECAg <ECAI.

Original languageEnglish
Pages (from-to)92-96
Number of pages5
Issue number2
Publication statusPublished - 1994


  • Adhesives
  • cell culture
  • cytotoxicity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering


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