TP53 and P16INK4A, but not H-KI-RAS, are involved in tumorigenesis and progression of pleomorphic adenomas

Claudia Augello, Valter Gregorio, Viviana Bazan, Patrizia Cammareri, Valentina Agnese, Sandra Cascio, Simona Corsale, Valentina Calò, Arianna Gullo, Rita Passantino, Grazia Gargano, Loredana Bruno, Gaetana Rinaldi, Vincenza Morello, Aldo Gerbino, Rosa Maria Tomasino, Marcella Macaluso, Eva Surmacz, Antonio Russo

Research output: Contribution to journalArticle

Abstract

The putative role of TP53 and p16INK4A tumor suppressor genes and Rasoncogenes in the development and progression of salivary gland neoplasias was studied in 28 cases of pleomorphic adenomas (PA), 4 cases of cystic adenocarcinomas, and 1 case of carcinoma ex-PA. Genetic and epigenetic alterations in the above genes were analyzed by Polymerase Chain Reaction/Single Strand Conformational Polymorphism (PCR/SSCP) and sequencing and by Methylation Specific-PCR (MS-PCR). Mutations in TP53 were found in 14% (4/28) of PAs and in 60% (3/5) of carcinomas. Mutations in H-Ras and K-Ras were identified in 4% (1/28) and 7% (2/28) of PAs, respectively. Only 20% (1/5) of carcinomas screened displayed mutations in K-Ras. p16INK4A promoter hypermethylation was found in 14% (4/28) of PAs and 100% (5/5) carcinomas. All genetic and epigenetic alterations were detected exclusively in the epithelial and transitional tumor components, and were absent in the mesenchymal parts. Our analysis suggests that TP53 mutations and p16INK4A promoter methylation, but not alterations in the H-Ras and K-Ras genes, might be involved in the malignant progression of PA into carcinoma.

Original languageEnglish
Pages (from-to)654-659
Number of pages6
JournalJournal of Cellular Physiology
Volume207
Issue number3
DOIs
Publication statusPublished - Jun 2006

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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    Augello, C., Gregorio, V., Bazan, V., Cammareri, P., Agnese, V., Cascio, S., Corsale, S., Calò, V., Gullo, A., Passantino, R., Gargano, G., Bruno, L., Rinaldi, G., Morello, V., Gerbino, A., Tomasino, R. M., Macaluso, M., Surmacz, E., & Russo, A. (2006). TP53 and P16INK4A, but not H-KI-RAS, are involved in tumorigenesis and progression of pleomorphic adenomas. Journal of Cellular Physiology, 207(3), 654-659. https://doi.org/10.1002/jcp.20601