Trabecular titanium can induce in vitro osteogenic differentiation of human adipose derived stem cells without osteogenic factors

Francesco Benazzo, Laura Botta, Manuela Federica Scaffino, Laura Caliogna, Matteo Marullo, Simonetta Fusi, Giulia Gastaldi

Research output: Contribution to journalArticle


Trabecular Titanium (TT) is an innovative highly porous structure that imitates the morphology of trabecular bone with good mechanical properties. Adipose-derived stem cells are a multipotent cell population that can be used in regenerative medicine, in particular, for bone therapeutic applications. The ability of TT to induce the osteogenic differentiation of human adipose derived stem cells (hASCs) in the absence of osteogenic factors was evaluated using molecular biological, biochemical, and immunohistochemical methods. At 7 and 21 days from differentiation, the hASCs grown on TT scaffolds showed similar expressions of alkaline phosphatase (ALP) and Runx-2 both in the presence and in the absence of osteogenic factors, as well as at transcript and protein levels. hASCs cultured on monolayer in the presence of the medium obtained from the wells where hASCs/scaffold constructs were cultured in the absence of osteogenic factors differentiated towards the osteogenic phenotype: their gene and protein expression of ALP and Runx-2 was similar to that of the same cells cultured in the presence of osteogenic factors, and significantly higher than that of the ones cultured in growth medium.

Original languageEnglish
Pages (from-to)2061-2071
Number of pages11
JournalJournal of Biomedical Materials Research - Part A
Issue number7
Publication statusPublished - 2014



  • alkaline phosphatase expression
  • human adipose derived stem cell
  • osteoblast-like cell
  • osteogenic differentiation
  • Runx-2 expression
  • trabecular titanium scaffold

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

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