Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors

Rohit Sachdeva, Marie E. Jönsson, Jenny Nelander, Agnete Kirkeby, Carolina Guibentif, Bernhard Gentner, Luigi Naldini, Anders Björklund, Malin Parmar, Johan Jakobsson

Research output: Contribution to journalArticle

Abstract

In this study, we have used a microRNA-regulated lentiviral reporter system to visualize and segregate differentiating neuronal cells in pluripotent cultures. Efficient suppression of transgene expression, specifically in undifferentiated pluripotent cells, was achieved by using a lentiviral vector expressing a fluorescent reporter gene regulated by microRNA-292. Using this strategy, it was possible to track progeny from murine ES, human ES cells, and induced pluripotent stem cells as they differentiated toward the neural lineage. In addition, this strategy was successfully used to FACS purify neuronal progenitors for molecular analysis and transplantation. FACS enrichment reduced tumor formation and increased survival of ES cell-derived neuronal progenitors after transplantation. The properties and versatility of the microRNA-regulated vectors allows broad use of these vectors in stem cell applications.

Original languageEnglish
Pages (from-to)11602-11607
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number25
DOIs
Publication statusPublished - Jun 22 2010

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Keywords

  • ES
  • Induced pluripotent stem cells
  • Stem cells
  • Transplantation

ASJC Scopus subject areas

  • General

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