Tractographic reconstruction protocol optimization in the rat brain in-vivo: Towards a normal atlas

Maria Giulia Preti, Alessandro Di Marzio, Alfonso Mastropietro, Domenico Aquino, Giuseppe Baselli, Maria Marcella Laganà, Ileana Zucca, Carolina Frassoni, Roberto Spreafico

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The tractographic reconstruction of anatomical and microstructural features provided by Magnetic Resonance (MR) Diffusion Tensor Imaging (DTI) gives essential information of brain damage in several pathological animal models. The optimization of a tractographic protocol is undertaken in normal rats for the future construction of a reference atlas, as prerequisite for preclinical pathological in-vivo studies. High field, preclinical in-vivo DTI faces important difficulties relevant to Signal-to-Noise Ratio (SNR), distortion, high required resolution, movement sensitivity. Given a pixel-size of 0.17 mm and TE/TR 29/6500 ms, b value and slice thickness were fixed at 700 s/mm 2 and 0.58 mm, respectively, on preventive ex-vivo studies. In-vivo studies led to the choice of 30 diffusion directions, averaged on 16 runs. The final protocol required 51 min scanning and permitted a reliable reconstruction of main rat brain bundles. Tract reconstruction stopping rules required proper setting. In conclusion, the viability of DTI tractography on in-vivo rat studies was shown, towards the construction of a normal reference atlas.

Original languageEnglish
Title of host publicationProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Pages8467-8470
Number of pages4
DOIs
Publication statusPublished - 2011
Event33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 - Boston, MA, United States
Duration: Aug 30 2011Sep 3 2011

Other

Other33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
CountryUnited States
CityBoston, MA
Period8/30/119/3/11

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

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  • Cite this

    Preti, M. G., Di Marzio, A., Mastropietro, A., Aquino, D., Baselli, G., Laganà, M. M., Zucca, I., Frassoni, C., & Spreafico, R. (2011). Tractographic reconstruction protocol optimization in the rat brain in-vivo: Towards a normal atlas. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (pp. 8467-8470). [6092089] https://doi.org/10.1109/IEMBS.2011.6092089