TRAIL promotes the survival and proliferation of primary human vascular endothelial cells by activating the Akt and ERK pathways

Paola Secchiero, Arianna Gonelli, Edvige Carnevale, Daniela Milani, Assunta Pandolfi, Davide Zella, Giorgio Zauli

Research output: Contribution to journalArticle

236 Citations (Scopus)

Abstract

Background - TRAIL protein is expressed in the medial smooth cell layer of aorta and pulmonary artery, whereas endothelial cells express all TRAIL receptors (TRAIL-Rs). Methods and Results - The role of TRAIL/TRAIL-Rs in vascular biology was investigated in primary human umbilical vein endothelial cells (HUVECs) and aortic endothelial cells, which showed comparable surface expression of death (TRAIL-R1 and -R2) and decoy (TRAIL-R3 and -R4) TRAIL-Rs. TRAIL activated the protein kinase Akt in HUVECs, as assessed by Western blot for phospho-Akt. Moreover, experiments performed with a pharmacological inhibitor of the phosphatidylinositol 3-kinase/Akt pathway (LY294002) or a dominant-negative Akt (K179M) demonstrated that TRAIL significantly protected HUVECs from apoptosis induced by trophic withdrawal via Akt and that inhibition of Akt sensitized HUVECs to TRAIL-induced caspase-dependent apoptosis. TRAIL also stimulated the ERK1/2 but not the p38 or the JNK pathways and induced a significant increase in endothelial cell proliferation in an ERK-dependent manner. Conversely, TRAIL did not activate NF-κB or affect the surface expression of the inflammatory markers E-selectin, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1. Conclusions - The ability of TRAIL to promote the survival/proliferation of endothelial cells without inducing NF-κB activation and inflammatory markers suggests that the TRAIL/TRAIL-R system plays an important role in endothelial cell physiology.

Original languageEnglish
Pages (from-to)2250-2256
Number of pages7
JournalCirculation
Volume107
Issue number17
DOIs
Publication statusPublished - May 6 2003

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MAP Kinase Signaling System
Human Umbilical Vein Endothelial Cells
Endothelial Cells
Survival
TNF-Related Apoptosis-Inducing Ligand
TNF-Related Apoptosis-Inducing Ligand Receptors
Phosphatidylinositol 3-Kinase
Apoptosis
Cell Physiological Phenomena
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
E-Selectin
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Caspases
Protein Kinases
Pulmonary Artery
Blood Vessels
Aorta
Western Blotting
Cell Proliferation

Keywords

  • Endothelium
  • Inflammation
  • Signal transduction

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

TRAIL promotes the survival and proliferation of primary human vascular endothelial cells by activating the Akt and ERK pathways. / Secchiero, Paola; Gonelli, Arianna; Carnevale, Edvige; Milani, Daniela; Pandolfi, Assunta; Zella, Davide; Zauli, Giorgio.

In: Circulation, Vol. 107, No. 17, 06.05.2003, p. 2250-2256.

Research output: Contribution to journalArticle

Secchiero, P, Gonelli, A, Carnevale, E, Milani, D, Pandolfi, A, Zella, D & Zauli, G 2003, 'TRAIL promotes the survival and proliferation of primary human vascular endothelial cells by activating the Akt and ERK pathways', Circulation, vol. 107, no. 17, pp. 2250-2256. https://doi.org/10.1161/01.CIR.0000062702.60708.C4
Secchiero, Paola ; Gonelli, Arianna ; Carnevale, Edvige ; Milani, Daniela ; Pandolfi, Assunta ; Zella, Davide ; Zauli, Giorgio. / TRAIL promotes the survival and proliferation of primary human vascular endothelial cells by activating the Akt and ERK pathways. In: Circulation. 2003 ; Vol. 107, No. 17. pp. 2250-2256.
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AU - Zella, Davide

AU - Zauli, Giorgio

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AB - Background - TRAIL protein is expressed in the medial smooth cell layer of aorta and pulmonary artery, whereas endothelial cells express all TRAIL receptors (TRAIL-Rs). Methods and Results - The role of TRAIL/TRAIL-Rs in vascular biology was investigated in primary human umbilical vein endothelial cells (HUVECs) and aortic endothelial cells, which showed comparable surface expression of death (TRAIL-R1 and -R2) and decoy (TRAIL-R3 and -R4) TRAIL-Rs. TRAIL activated the protein kinase Akt in HUVECs, as assessed by Western blot for phospho-Akt. Moreover, experiments performed with a pharmacological inhibitor of the phosphatidylinositol 3-kinase/Akt pathway (LY294002) or a dominant-negative Akt (K179M) demonstrated that TRAIL significantly protected HUVECs from apoptosis induced by trophic withdrawal via Akt and that inhibition of Akt sensitized HUVECs to TRAIL-induced caspase-dependent apoptosis. TRAIL also stimulated the ERK1/2 but not the p38 or the JNK pathways and induced a significant increase in endothelial cell proliferation in an ERK-dependent manner. Conversely, TRAIL did not activate NF-κB or affect the surface expression of the inflammatory markers E-selectin, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1. Conclusions - The ability of TRAIL to promote the survival/proliferation of endothelial cells without inducing NF-κB activation and inflammatory markers suggests that the TRAIL/TRAIL-R system plays an important role in endothelial cell physiology.

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