Trans-Activation of Epidermal Growth Factor Receptor Gene by the Hepatitis B Virus X-Gene Product

Stefano Menzo, Massimo Clementi, Elena Alfani, Patrizia Bagnarelli, Silvia Iacovacci, Aldo Manzin, Maura Dandri, Gioacchino Natoli, Massimo Levrero, Guido Carloni

Research output: Contribution to journalArticlepeer-review


The expression of the cellular gene coding for the epidermal growth factor (EGF) receptor (EGF-R) was assayed in the presence of hepatitis B virus (HBV) gene expression under different experimental conditions in human hepatoma-derived cells. First, transfection experiments of the well-differentiated HepG2 human hepatoma cell line using different expression vectors of the HBV X-region demonstrated that the X-gene product is capable of inducing EGF-R gene overexpression; in addition, by using a stable in vitro expression system for HBV, it was shown that EGF-R gene expression in these cells is greater than in the uninfected parent cells, and that this results in a three-fold increase in 125I-EGF binding. Finally, a CAT-expression assay was performed, indicating that regulatory regions of the EGF-R-gene are target sequences for X-protein trans-activation.

Original languageEnglish
Pages (from-to)878-882
Number of pages5
Issue number2
Publication statusPublished - Oct 1993

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology


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