TY - JOUR
T1 - Trans-Activation of Epidermal Growth Factor Receptor Gene by the Hepatitis B Virus X-Gene Product
AU - Menzo, Stefano
AU - Clementi, Massimo
AU - Alfani, Elena
AU - Bagnarelli, Patrizia
AU - Iacovacci, Silvia
AU - Manzin, Aldo
AU - Dandri, Maura
AU - Natoli, Gioacchino
AU - Levrero, Massimo
AU - Carloni, Guido
PY - 1993/10
Y1 - 1993/10
N2 - The expression of the cellular gene coding for the epidermal growth factor (EGF) receptor (EGF-R) was assayed in the presence of hepatitis B virus (HBV) gene expression under different experimental conditions in human hepatoma-derived cells. First, transfection experiments of the well-differentiated HepG2 human hepatoma cell line using different expression vectors of the HBV X-region demonstrated that the X-gene product is capable of inducing EGF-R gene overexpression; in addition, by using a stable in vitro expression system for HBV, it was shown that EGF-R gene expression in these cells is greater than in the uninfected parent cells, and that this results in a three-fold increase in 125I-EGF binding. Finally, a CAT-expression assay was performed, indicating that regulatory regions of the EGF-R-gene are target sequences for X-protein trans-activation.
AB - The expression of the cellular gene coding for the epidermal growth factor (EGF) receptor (EGF-R) was assayed in the presence of hepatitis B virus (HBV) gene expression under different experimental conditions in human hepatoma-derived cells. First, transfection experiments of the well-differentiated HepG2 human hepatoma cell line using different expression vectors of the HBV X-region demonstrated that the X-gene product is capable of inducing EGF-R gene overexpression; in addition, by using a stable in vitro expression system for HBV, it was shown that EGF-R gene expression in these cells is greater than in the uninfected parent cells, and that this results in a three-fold increase in 125I-EGF binding. Finally, a CAT-expression assay was performed, indicating that regulatory regions of the EGF-R-gene are target sequences for X-protein trans-activation.
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U2 - 10.1006/viro.1993.1550
DO - 10.1006/viro.1993.1550
M3 - Article
C2 - 8396816
AN - SCOPUS:0027448556
VL - 196
SP - 878
EP - 882
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -