Abstract
In this report we investigate the signalling pathway activated by H2O2 in human adenocarcinoma gastric cells (AGS) and we evaluate the anti-proliferative action of the natural stilbene trans-resveratrol. We demonstrate that H2O2 accelerates cell growth and induces a prompt MEK1/2-ERK1/2 activation. Such events are also associated with the activation of c-Jun and its translocation into the nuclear compartment. A specific inhibitor of ERK1/2 phosphorylation by MEK1/2 (U0126) abrogates these phenomena. On the contrary, specific inhibition of JNK activity does not influence H2O2-mediated growth, suggesting that cell proliferation likely proceeds via MEK1/2-ERK1/2-Jun signalling axis. trans-Resveratrol is also able to completely suppress the increase in proliferation. We demonstrate that this property is not due to its antioxidant capacity but rather due to a specific inhibition of ERK1/2 phosphorylation by MEK1/2 and repression of c-Jun activation.
Original language | English |
---|---|
Pages (from-to) | 337-347 |
Number of pages | 11 |
Journal | Biochemical Pharmacology |
Volume | 77 |
Issue number | 3 |
DOIs | |
Publication status | Published - Feb 1 2009 |
Keywords
- c-Jun
- Cell proliferation
- ERK1/2
- Hydrogen peroxide
- Resveratrol
ASJC Scopus subject areas
- Pharmacology
- Biochemistry