Transabdominal coelocentesis as early source of fetal DNA for chromosomal and molecular diagnosis

Adalgisa Pietropolli, Roberta Vicario, Cristina Peconi, Stefania Zampatti, Maria Chiara Quitadamo, Maria Vittoria Capogna, Michele Ragazzo, Anna Maria Nardone, Diana Postorivo, Paola Spitalieri, Simona Sarta, Fabrizio Ratto, Giuseppe Novelli, Federica Sangiuolo, Emilio Piccione, Emiliano Giardina

Research output: Contribution to journalArticle

Abstract

This study reports a comparative analysis between results of transabdominal coelocentesis and traditional invasive procedure in order to assess the usefulness of coelocentesis as a source of fetal DNA for molecular and chromosomal analysis. A number of 28 women were included in the study. A successful sampling of coelomic fluid was obtained in 25 women by transabdominal procedure. A positive amplification of DNA with QF-PCR techniques was obtained in 90% of cases, while 10% of cases failed to reveal interpretable results. Although all samples were cultured, the growth rate was not sufficient to determine karyotypes within 2 weeks. Five samples were selected to be analyzed by array-based comparative genomic hybridization (a-CGH) but the interpretation of these results was difficult and ambiguous. Our results suggest that transabdominal coelocentesis is suitable for the detection of single DNA variation and for QF-PCR analysis, while further experiments are needed to develop optimized protocols for traditional karyotyping and array-analysis.

Original languageEnglish
Pages (from-to)1656-1660
Number of pages5
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume27
Issue number16
DOIs
Publication statusPublished - Nov 1 2014

Keywords

  • Array-based comparative genomic hybridization
  • Coelomic fluid
  • Fetal DNA
  • QF-PCR
  • Transabdominal coelocentesis

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynaecology
  • Medicine(all)

Fingerprint Dive into the research topics of 'Transabdominal coelocentesis as early source of fetal DNA for chromosomal and molecular diagnosis'. Together they form a unique fingerprint.

  • Cite this

    Pietropolli, A., Vicario, R., Peconi, C., Zampatti, S., Quitadamo, M. C., Capogna, M. V., Ragazzo, M., Nardone, A. M., Postorivo, D., Spitalieri, P., Sarta, S., Ratto, F., Novelli, G., Sangiuolo, F., Piccione, E., & Giardina, E. (2014). Transabdominal coelocentesis as early source of fetal DNA for chromosomal and molecular diagnosis. Journal of Maternal-Fetal and Neonatal Medicine, 27(16), 1656-1660. https://doi.org/10.3109/14767058.2013.871697