Transcript profiling of chitosan-treated Arabidopsis seedlings

Giovanni Povero, Elena Loreti, Chiara Pucciariello, Antonietta Santaniello, Donata Di Tommaso, Gianluca Di Tommaso, Dimos Kapetis, Francesca Zolezzi, Alberto Piaggesi, Pierdomenico Perata

Research output: Contribution to journalArticle

Abstract

In nature, plants can recognize potential pathogens, thus activating intricate networks of defense signals and reactions. Inducible defense is often mediated by the detection of microbe or pathogen associated molecular pattern elicitors, such as flagellin and chitin. Chitosan, the deacetylated form of chitin, plays a role in inducing protection against pathogens in many plant species. We evaluated the ability of chitosan to confer resistance to Botrytis cinerea in Arabidopsis leaves. We subsequently treated Arabidopsis seedlings with chitosan and carried out a transcript profiling analysis using both ATH1 GeneChip microarrays and quantitative RT-PCR. The results showed that defense response genes, including camalexin biosynthesis genes, were up-regulated by chitosan, both in wild-type and in the chitin-insensitive cerk1 mutant, indicating that chitosan is perceived through a CERK1-independent pathway.

Original languageEnglish
Pages (from-to)619-629
Number of pages11
JournalJournal of Plant Research
Volume124
Issue number5
DOIs
Publication statusPublished - Sep 2011

Keywords

  • Arabidopsis
  • Botrytis cinerea
  • CERK1
  • Chitosan
  • Microarray
  • Transcript profiling

ASJC Scopus subject areas

  • Plant Science

Fingerprint Dive into the research topics of 'Transcript profiling of chitosan-treated Arabidopsis seedlings'. Together they form a unique fingerprint.

  • Cite this

    Povero, G., Loreti, E., Pucciariello, C., Santaniello, A., Di Tommaso, D., Di Tommaso, G., Kapetis, D., Zolezzi, F., Piaggesi, A., & Perata, P. (2011). Transcript profiling of chitosan-treated Arabidopsis seedlings. Journal of Plant Research, 124(5), 619-629. https://doi.org/10.1007/s10265-010-0399-1