Transcription Factor EB Controls Metabolic Flexibility during Exercise

Gelsomina Mansueto, Andrea Armani, Carlo Viscomi, Carlo Viscomi, Luca D'Orsi, Rossella De Cegli, Elena V. Polishchuk, Costanza Lamperti, Ivano Di Meo, Vanina Romanello, Vanina Romanello, Silvia Marchet, Pradip K. Saha, Haihong Zong, Haihong Zong, Bert Blaauw, Francesca Solagna, Caterina Tezze, Paolo Grumati, Paolo BonaldoJeffrey E. Pessin, Massimo Zeviani, Massimo Zeviani, Marco Sandri, Marco Sandri, Marco Sandri, Andrea Ballabio, Andrea Ballabio, Andrea Ballabio, Andrea Ballabio

Research output: Contribution to journalArticlepeer-review


© 2017 The Authors The transcription factor EB (TFEB) is an essential component of lysosomal biogenesis and autophagy for the adaptive response to food deprivation. To address the physiological function of TFEB in skeletal muscle, we have used muscle-specific gain- and loss-of-function approaches. Here, we show that TFEB controls metabolic flexibility in muscle during exercise and that this action is independent of peroxisome proliferator-activated receptor-γ coactivator1α (PGC1α). Indeed, TFEB translocates into the myonuclei during physical activity and regulates glucose uptake and glycogen content by controlling expression of glucose transporters, glycolytic enzymes, and pathways related to glucose homeostasis. In addition, TFEB induces the expression of genes involved in mitochondrial biogenesis, fatty acid oxidation, and oxidative phosphorylation. This coordinated action optimizes mitochondrial substrate utilization, thus enhancing ATP production and exercise capacity. These findings identify TFEB as a critical mediator of the beneficial effects of exercise on metabolism.
Original languageEnglish
Pages (from-to)182-196
Number of pages15
JournalCell Metabolism
Issue number1
Publication statusPublished - Jan 10 2017


  • autophagy
  • diabetes
  • exercise
  • glucose
  • insulin
  • metabolic flexibility
  • mitochondria
  • mitochondrial fusion
  • PGC1alpha
  • TFEB


Dive into the research topics of 'Transcription Factor EB Controls Metabolic Flexibility during Exercise'. Together they form a unique fingerprint.

Cite this