Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation

Aymone Gurtner, Paola Fuschi, Fabio Martelli, Isabella Manni, Simona Artuso, Giacoma Simonte, Valeria Ambrosino, Annalisa Antonini, Valentina Folgiero, Rita Falcioni, Ada Sacchi, Giulia Piaggio

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The CCAAT-binding transcription factor NF-Y plays a central role in regulating cellular proliferation by controlling the expression of genes required for cell-cycle progression such as cyclin A, cyclin B1, cyclin B2, cdc25A, cdc25C, and cdk1. Here we show that unrestricted NF-Y activity leads to apoptosis in an E2F1- and wild-type p53 (wtp53)-dependent manner. Unrestricted NF-Y activity induced an increase in E2F1 mRNA and protein levels. Furthermore, NF-Y directly bound the E2F1 promoter and this correlated with the appearance of open chromatin marks. The ability of NF-Y to induce apoptosis was impaired in cells lacking E2F1 and wtp53. Moreover, NF-Y overexpression elicited phosphorylation of wt p53Ser18 in an E2F1-dependent manner. Our findings establish that NF-Y acts upstream of E2F1 in p53-mediated apoptosis.

Original languageEnglish
Pages (from-to)9711-9720
Number of pages10
JournalCancer Research
Volume70
Issue number23
DOIs
Publication statusPublished - Dec 1 2010

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Transcription Factors
Up-Regulation
Apoptosis
Cyclin B2
CCAAT-Binding Factor
Cyclin B1
Cyclin A
cdc Genes
Chromatin
Phosphorylation
Cell Proliferation
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation. / Gurtner, Aymone; Fuschi, Paola; Martelli, Fabio; Manni, Isabella; Artuso, Simona; Simonte, Giacoma; Ambrosino, Valeria; Antonini, Annalisa; Folgiero, Valentina; Falcioni, Rita; Sacchi, Ada; Piaggio, Giulia.

In: Cancer Research, Vol. 70, No. 23, 01.12.2010, p. 9711-9720.

Research output: Contribution to journalArticle

Gurtner, Aymone ; Fuschi, Paola ; Martelli, Fabio ; Manni, Isabella ; Artuso, Simona ; Simonte, Giacoma ; Ambrosino, Valeria ; Antonini, Annalisa ; Folgiero, Valentina ; Falcioni, Rita ; Sacchi, Ada ; Piaggio, Giulia. / Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation. In: Cancer Research. 2010 ; Vol. 70, No. 23. pp. 9711-9720.
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