Transcription of a T cell receptor β chain gene in L cell fibroblasts following DNA-mediated gene transfer

M. Sensi, Z. Dembic, M. Steinmetz

Research output: Contribution to journalArticlepeer-review

Abstract

Functionally rearranged T cell receptor α and β-chain genes from a fluorescein-specific cytotoxic T cell clone have been introduced, together with the selectable marker gene neo, into mouse fibroblasts (L cells) by electroporation. Transformed cells were selected for neo gene expression by growth in medium containing the antibiotic G418. Southern blot analysis of DNA from transformed L cell clones revealed that the endogeneous T cell receptor α and β-chain genes were in germ-line configuration and that in 4 of the 6 clones examined the exogenously introduced rearranged α and β chain genes were present. The introduced β-chain gene is transcriptionally active in two L cell clones examined whereas no transcription of the α-chain gene could be detected in the same transformants.

Original languageEnglish
Pages (from-to)1371-1374
Number of pages4
JournalEuropean Journal of Immunology
Volume17
Issue number9
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Immunology

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