Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site

Rosario Ammendola, Fotini Gounari, Giulia Piaggio, Vincenzo De Simone, Riccardo Cortese

Research output: Contribution to journalArticle

Abstract

The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.

Original languageEnglish
Pages (from-to)387-390
Number of pages4
JournalMolecular and Cellular Biology
Volume10
Issue number1
Publication statusPublished - Jan 1990

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Sp1 Transcription Factor
Chloramphenicol O-Acetyltransferase
Transcription Initiation Site
Hep G2 Cells
Plasmids
Transcription Factors
Gels
Binding Sites
Cell Line
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site. / Ammendola, Rosario; Gounari, Fotini; Piaggio, Giulia; De Simone, Vincenzo; Cortese, Riccardo.

In: Molecular and Cellular Biology, Vol. 10, No. 1, 01.1990, p. 387-390.

Research output: Contribution to journalArticle

Ammendola, Rosario ; Gounari, Fotini ; Piaggio, Giulia ; De Simone, Vincenzo ; Cortese, Riccardo. / Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site. In: Molecular and Cellular Biology. 1990 ; Vol. 10, No. 1. pp. 387-390.
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