Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site

Rosario Ammendola, Fotini Gounari, Giulia Piaggio, Vincenzo De Simone, Riccardo Cortese

Research output: Contribution to journalArticlepeer-review

Abstract

The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.

Original languageEnglish
Pages (from-to)387-390
Number of pages4
JournalMolecular and Cellular Biology
Volume10
Issue number1
Publication statusPublished - Jan 1990

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site'. Together they form a unique fingerprint.

Cite this