Transcription signals in embryonic Xenopus laevis U1 RNA genes.

G. Ciliberto, R. Buckland, R. Cortese, L. Philipson

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A genomic clone of the most abundant U1 RNA genes from Xenopus laevis was isolated from erythrocyte DNA and sequenced. Two different U1 RNA genes, U1A and U1B, are encoded in an HindIII 1.5-kb fragment and both are expressed after microinjection in Xenopus oocytes. Deletions and site-directed mutagenesis of the clones revealed two promoter elements in the U1B gene; one, located 250-220 nucleotides upstream from the 5' terminus of mature U1 RNA, functions as an activator, yielding a 10-fold promotion of transcription; the other, located 60-50 nucleotides upstream of the cap site, functions as an essential element for promotion of transcription. The U1A gene contained only the latter element in the cloned fragment. Homologous sequences can be identified in several U RNA genes of X. laevis.

Original languageEnglish
Pages (from-to)1537-1543
Number of pages7
JournalEMBO Journal
Volume4
Issue number6
Publication statusPublished - Jun 1985

Fingerprint

Xenopus laevis
Transcription
Genes
Nucleotides
Clone Cells
Activator Appliances
Mutagenesis
Microinjections
Sequence Homology
Xenopus
Site-Directed Mutagenesis
Oocytes
Erythrocytes
U1 small nuclear RNA
RNA
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Ciliberto, G., Buckland, R., Cortese, R., & Philipson, L. (1985). Transcription signals in embryonic Xenopus laevis U1 RNA genes. EMBO Journal, 4(6), 1537-1543.

Transcription signals in embryonic Xenopus laevis U1 RNA genes. / Ciliberto, G.; Buckland, R.; Cortese, R.; Philipson, L.

In: EMBO Journal, Vol. 4, No. 6, 06.1985, p. 1537-1543.

Research output: Contribution to journalArticle

Ciliberto, G, Buckland, R, Cortese, R & Philipson, L 1985, 'Transcription signals in embryonic Xenopus laevis U1 RNA genes.', EMBO Journal, vol. 4, no. 6, pp. 1537-1543.
Ciliberto G, Buckland R, Cortese R, Philipson L. Transcription signals in embryonic Xenopus laevis U1 RNA genes. EMBO Journal. 1985 Jun;4(6):1537-1543.
Ciliberto, G. ; Buckland, R. ; Cortese, R. ; Philipson, L. / Transcription signals in embryonic Xenopus laevis U1 RNA genes. In: EMBO Journal. 1985 ; Vol. 4, No. 6. pp. 1537-1543.
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