Transcriptional and post-transcriptional regulation of IL-1β, IL-6 and TNF-α genes in chronic lymphocytic leukaemia

A. Rambaldi, S. Bettoni, V. Rossi, M. L. Tini, G. Giudici, V. Rizzo, R. Bassan, A. Mantovani, T. Barbui, A. Biondi

Research output: Contribution to journalArticle

Abstract

The present study was designed to define the mechanisms of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumour necrosis factor (TNF-α) gene regulation in chronic lymphocytic leukaemia of B cell origin (B-CLL). By nuclear run-on analysis, all B-CLL cases displayed high levels of nuclear transcription of the IL-6 and TNF-α genes, whereas IL-1β gene transcription was only barely detectable. Upon in vitro culture for 1 h, B-CLL cells from different patients were substantially heterogeneous in terms of expression of steady state mRNA levels of IL-1β, IL-6 and TNF-α even though the pattern of nuclear transcription of these cytokines was only marginally affected by in vitro culture. mRNA stability was then examined and cytokine gene transcripts showed a half life of more than 2 h in cultured B-CLL cells and treatment with cycloheximide (CHX) did not affect cytokine transcript levels in B-CLL cells. These results indicate that: steady state levels of each mRNA do not reflect the rate of nuclear transcription of these cytokines in fresh or cultured B-CLL cells, that purification and in vitro culture of leukaemic cells may amplify cytokine gene expression in B-CLL, and that cytokine gene transcripts are relatively stable in B-CLL.

Original languageEnglish
Pages (from-to)204-211
Number of pages8
JournalBritish Journal of Haematology
Volume83
Issue number2
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Hematology

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    Rambaldi, A., Bettoni, S., Rossi, V., Tini, M. L., Giudici, G., Rizzo, V., Bassan, R., Mantovani, A., Barbui, T., & Biondi, A. (1993). Transcriptional and post-transcriptional regulation of IL-1β, IL-6 and TNF-α genes in chronic lymphocytic leukaemia. British Journal of Haematology, 83(2), 204-211.