Transcriptional coactivator EDF-1 is required for PPARγ-stimulated adipogenesis

Marzia Leidi, Massimo Mariotti, J. A M Maier

Research output: Contribution to journalArticlepeer-review

Abstract

Peroxisome proliferator-activated receptor-γ (PPARγ) is essential for adipogenesis. Since EDF-1 is a cofactor of PPARγ, we investigated the molecular cross-talk between EDF-1 and PPARγ in adipogenesis. While EDF-1 was not modulated during differentiation of 3T3-L1 cells, it co-immunoprecipitated with PPARγ. Silencing EDF-1 by shRNAs inhibited the differentiation in adipocytes of 3T3-L1 cells, as detected by the staining of intracellular triglycerides and the expression of the PPARγ target gene aP2. Accordingly, we found that anti-EDF-1 shRNAs decreased ligand dependent activation of PPARγ in 3T3-L1 transiently transfected with a vector expressing luciferase under the control of a PPARγ responsive consensus. To rule out that this inhibition is due to the concomitant downregulation of PPARγ levels, we overexpressed PPARγ in 3T3-L1 silencing EDF-1 and found a decrease of ligand dependent activation of PPARγ, in spite of the high amounts of PPARγ. These results demonstrate that EDF-1 is required for PPARγ transcriptional activation during 3T3-L1 differentiation.

Original languageEnglish
Pages (from-to)2733-2742
Number of pages10
JournalCellular and Molecular Life Sciences
Volume66
Issue number16
DOIs
Publication statusPublished - Aug 2009

Keywords

  • Adipogenesis
  • EDF-1
  • PPARγ
  • Transcriptional coactivator

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Medicine(all)
  • Cellular and Molecular Neuroscience
  • Pharmacology

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