Abstract
Peroxisome proliferator-activated receptor-γ (PPARγ) is essential for adipogenesis. Since EDF-1 is a cofactor of PPARγ, we investigated the molecular cross-talk between EDF-1 and PPARγ in adipogenesis. While EDF-1 was not modulated during differentiation of 3T3-L1 cells, it co-immunoprecipitated with PPARγ. Silencing EDF-1 by shRNAs inhibited the differentiation in adipocytes of 3T3-L1 cells, as detected by the staining of intracellular triglycerides and the expression of the PPARγ target gene aP2. Accordingly, we found that anti-EDF-1 shRNAs decreased ligand dependent activation of PPARγ in 3T3-L1 transiently transfected with a vector expressing luciferase under the control of a PPARγ responsive consensus. To rule out that this inhibition is due to the concomitant downregulation of PPARγ levels, we overexpressed PPARγ in 3T3-L1 silencing EDF-1 and found a decrease of ligand dependent activation of PPARγ, in spite of the high amounts of PPARγ. These results demonstrate that EDF-1 is required for PPARγ transcriptional activation during 3T3-L1 differentiation.
Original language | English |
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Pages (from-to) | 2733-2742 |
Number of pages | 10 |
Journal | Cellular and Molecular Life Sciences |
Volume | 66 |
Issue number | 16 |
DOIs | |
Publication status | Published - Aug 2009 |
Keywords
- Adipogenesis
- EDF-1
- PPARγ
- Transcriptional coactivator
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Molecular Medicine
- Medicine(all)
- Cellular and Molecular Neuroscience
- Pharmacology