Transcriptional downregulation of tight junction protein ZO-1 in active coeliac disease is reversed after a gluten-free diet

D. Pizzuti, M. Bortolami, E. Mazzon, A. Buda, G. Guariso, A. D'Odorico, S. Chiarelli, R. D'Incà, F. De Lazzari, D. Martines

Research output: Contribution to journalArticlepeer-review


Coeliac disease is an autoimmune enteropathy characterized by an enhanced permeability of the intestinal epithelial barrier. In epithelial cells paracellular permeability is regulated by intercellular tight junction. The cytoplasmic protein ZO-1 interacts directly with F-actin and plays a pivotal role in the structural and functional organization of tight junction. Aim. The aim of this study was to investigate the expression and localization of ZO-1 in the intestinal mucosa of coeliac patients. Patients and methods. Twenty patients with active coeliac disease, seven of whom underwent a repeat biopsy following a gluten-free diet and 27 control subjects, were studied. In all subjects, three biopsies were obtained from distal duodenum during upper gastrointestinal endoscopy. ZO-1 protein localization and levels were detected by immunofluorescence followed by confocal microscopy analysis and immunoblotting. ZO-1 mRNA expression was assessed by RT-PCR. F-actin distribution was also investigated. Results. In patients with active coeliac disease, both ZO-1 protein levels and mRNA were clearly reduced. Cytoskeletal organization was disrupted with F-actin staining concentrated at the subcortical and basal surface regions. Abnormalities in ZO-1 expression and actin organization were reversed after a gluten-free diet. Conclusions. In active coeliac disease, ZO-1 protein expression is downregulated at the transcriptional level in association with F-actin redistribution. These changes are completely reversed after a gluten-free diet and could contribute to the increased intestinal paracellular permeability observed in this disorder.

Original languageEnglish
Pages (from-to)337-341
Number of pages5
JournalDigestive and Liver Disease
Issue number5
Publication statusPublished - May 2004


  • Coeliac disease
  • Gluten-free diet
  • Tight junction

ASJC Scopus subject areas

  • Gastroenterology


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