Transcriptional factors for epithelial-mesenchymal transition are associated with mesenchymal differentiation in gliosarcoma

Masaya Nagaishi, Werner Paulus, Benjamin Brokinkel, Anne Vital, Yuko Tanaka, Yoichi Nakazato, Felice Giangaspero, Hiroko Ohgaki

Research output: Contribution to journalArticlepeer-review

Abstract

Gliosarcoma is a rare variant of glioblastoma characterized by a biphasic pattern of glial and mesenchymal differentiation. It is unclear whether mesenchymal differentiation in gliosarcomas is because of extensive genomic instability and/or to a mechanism similar to epithelial-mesenchymal transition (EMT). In the present study, we assessed 40 gliosarcomas for immunoreactivity of Slug, Twist, matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), which are involved in EMT in epithelial tumors. Nuclear Slug expression was observed in >50% of neoplastic cells in mesenchymal tumor areas of 33 (83%) gliosarcomas, but not in glial areas (P <0.0001). Nuclear Twist expression was observed in >50% of neoplastic cells in mesenchymal tumor areas of 35 (88%) gliosarcomas, but glial tumor areas were largely negative except in four cases (P <0.0001). Expression of MMP-2 and MMP-9 was also significantly more extensive in mesenchymal than in glial tumor areas. None of 20 ordinary glioblastomas showed Slug or Twist expression in >10% neoplastic cells. Thus, expression of Slug, Twist, MMP-2 and MMP-9 was characteristic of mesenchymal tumor areas of gliosarcomas, suggesting that mechanisms involved in the EMT in epithelial neoplasms may play roles in mesenchymal differentiation in gliosarcomas.

Original languageEnglish
Pages (from-to)670-676
Number of pages7
JournalBrain Pathology
Volume22
Issue number5
DOIs
Publication statusPublished - Sep 2012

Keywords

  • epithelial to mesenchymal transition
  • gliosarcoma
  • MMP
  • Slug
  • Twist

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pathology and Forensic Medicine
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Transcriptional factors for epithelial-mesenchymal transition are associated with mesenchymal differentiation in gliosarcoma'. Together they form a unique fingerprint.

Cite this