Transcriptional profile of the immune response in the lungs of patients with active tuberculosis

Manuela Grassi, Marialuisa Bocchino, Almerico Marruchella, Elisabetta Volpe, Cesare Saltini, Vittorio Colizzi, Francesca Mariani

Research output: Contribution to journalArticle

Abstract

Despite advances in diagnosis and treatment, Mycobacterium tuberculosis causes active disease in about 8 million people worldwide annually. The study of the interplay between the host and the pathogen at the site of infection in human TB may contribute to elucidate the pathogenesis of the disease. In this work, using macroarray technology and real-time PCR, we analyzed the modulation of 847 genes encoding immune-inflammatory mediators in BALF samples of patients affected by active pulmonary TB (PTB) and control patients affected by non-TB diseases. The data show that the PTB milieu contains a complex network of gene activation. Different genes with adhesive properties and involved in tissue repair and fibrosis were modulated. In TB patients, we observed the up-regulation of cytokines, including IFN-γ and IFN-γ pathway genes, of several apoptotic genes, and of potent transcriptional activators. These findings can contribute to elucidate the mechanisms of MTB pathogenicity in humans.

Original languageEnglish
Pages (from-to)100-107
Number of pages8
JournalClinical Immunology
Volume121
Issue number1
DOIs
Publication statusPublished - Oct 2006

Keywords

  • BALF
  • Host-pathogen interaction
  • Immune response
  • Inflammatory cytokine genes
  • Macroarray
  • Mycobacterium tuberculosis
  • Site of infection

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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  • Cite this

    Grassi, M., Bocchino, M., Marruchella, A., Volpe, E., Saltini, C., Colizzi, V., & Mariani, F. (2006). Transcriptional profile of the immune response in the lungs of patients with active tuberculosis. Clinical Immunology, 121(1), 100-107. https://doi.org/10.1016/j.clim.2006.06.008