Transcriptional reprogramming of dendritic cells by differentiation stimuli

Francesca Granucci, Caterina Vizzardelli, Ettore Virzi, Maria Rescigno, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Immature and mature dendritic cells (DC) have been well characterized functionally and phenotypically. Microorganisms or bacterial products such as lipopolysaccharide (LPS) and inflammatory molecules, including tumor necrosis factor (TNF-α), are both believed to activate the DC maturation program which allows DC to initiate and amplify innate and adaptive immune responses. However, there is increasing evidence that the functional state of DC, induced by different stimuli, may be relevant for the immune response outcome. Thus, we compared the transcriptional program of mature, transitional and immature DC, after either LPS or TNF-α stimulation. GeneChip® oligonucleotide microarrays, representing approximately 6,500 murine genes and ESTs, were used for this analysis. A very diverse modulation of gene expression was observed with the two stimuli. Only LPS-treated cells showed a pattern of expression of genes compatible with a definitive growth arrest and with a suitable activation and control of the immune response.

Original languageEnglish
Pages (from-to)2539-2546
Number of pages8
JournalEuropean Journal of Immunology
Volume31
Issue number9
DOIs
Publication statusPublished - 2001

Fingerprint

Dendritic Cells
Cell Differentiation
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Gene Expression
Expressed Sequence Tags
Adaptive Immunity
Oligonucleotide Array Sequence Analysis
Innate Immunity
Growth
Genes

Keywords

  • Dendritic cell
  • Inflammatory stimulus
  • Microarray
  • Transcription analysis

ASJC Scopus subject areas

  • Immunology

Cite this

Transcriptional reprogramming of dendritic cells by differentiation stimuli. / Granucci, Francesca; Vizzardelli, Caterina; Virzi, Ettore; Rescigno, Maria; Ricciardi-Castagnoli, Paola.

In: European Journal of Immunology, Vol. 31, No. 9, 2001, p. 2539-2546.

Research output: Contribution to journalArticle

Granucci, Francesca ; Vizzardelli, Caterina ; Virzi, Ettore ; Rescigno, Maria ; Ricciardi-Castagnoli, Paola. / Transcriptional reprogramming of dendritic cells by differentiation stimuli. In: European Journal of Immunology. 2001 ; Vol. 31, No. 9. pp. 2539-2546.
@article{9ac9aca585b143b3b4d7ceed3853a281,
title = "Transcriptional reprogramming of dendritic cells by differentiation stimuli",
abstract = "Immature and mature dendritic cells (DC) have been well characterized functionally and phenotypically. Microorganisms or bacterial products such as lipopolysaccharide (LPS) and inflammatory molecules, including tumor necrosis factor (TNF-α), are both believed to activate the DC maturation program which allows DC to initiate and amplify innate and adaptive immune responses. However, there is increasing evidence that the functional state of DC, induced by different stimuli, may be relevant for the immune response outcome. Thus, we compared the transcriptional program of mature, transitional and immature DC, after either LPS or TNF-α stimulation. GeneChip{\circledR} oligonucleotide microarrays, representing approximately 6,500 murine genes and ESTs, were used for this analysis. A very diverse modulation of gene expression was observed with the two stimuli. Only LPS-treated cells showed a pattern of expression of genes compatible with a definitive growth arrest and with a suitable activation and control of the immune response.",
keywords = "Dendritic cell, Inflammatory stimulus, Microarray, Transcription analysis",
author = "Francesca Granucci and Caterina Vizzardelli and Ettore Virzi and Maria Rescigno and Paola Ricciardi-Castagnoli",
year = "2001",
doi = "10.1002/1521-4141(200109)31:9<2539::AID-IMMU2539>3.0.CO;2-9",
language = "English",
volume = "31",
pages = "2539--2546",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "wiley",
number = "9",

}

TY - JOUR

T1 - Transcriptional reprogramming of dendritic cells by differentiation stimuli

AU - Granucci, Francesca

AU - Vizzardelli, Caterina

AU - Virzi, Ettore

AU - Rescigno, Maria

AU - Ricciardi-Castagnoli, Paola

PY - 2001

Y1 - 2001

N2 - Immature and mature dendritic cells (DC) have been well characterized functionally and phenotypically. Microorganisms or bacterial products such as lipopolysaccharide (LPS) and inflammatory molecules, including tumor necrosis factor (TNF-α), are both believed to activate the DC maturation program which allows DC to initiate and amplify innate and adaptive immune responses. However, there is increasing evidence that the functional state of DC, induced by different stimuli, may be relevant for the immune response outcome. Thus, we compared the transcriptional program of mature, transitional and immature DC, after either LPS or TNF-α stimulation. GeneChip® oligonucleotide microarrays, representing approximately 6,500 murine genes and ESTs, were used for this analysis. A very diverse modulation of gene expression was observed with the two stimuli. Only LPS-treated cells showed a pattern of expression of genes compatible with a definitive growth arrest and with a suitable activation and control of the immune response.

AB - Immature and mature dendritic cells (DC) have been well characterized functionally and phenotypically. Microorganisms or bacterial products such as lipopolysaccharide (LPS) and inflammatory molecules, including tumor necrosis factor (TNF-α), are both believed to activate the DC maturation program which allows DC to initiate and amplify innate and adaptive immune responses. However, there is increasing evidence that the functional state of DC, induced by different stimuli, may be relevant for the immune response outcome. Thus, we compared the transcriptional program of mature, transitional and immature DC, after either LPS or TNF-α stimulation. GeneChip® oligonucleotide microarrays, representing approximately 6,500 murine genes and ESTs, were used for this analysis. A very diverse modulation of gene expression was observed with the two stimuli. Only LPS-treated cells showed a pattern of expression of genes compatible with a definitive growth arrest and with a suitable activation and control of the immune response.

KW - Dendritic cell

KW - Inflammatory stimulus

KW - Microarray

KW - Transcription analysis

UR - http://www.scopus.com/inward/record.url?scp=0034801738&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034801738&partnerID=8YFLogxK

U2 - 10.1002/1521-4141(200109)31:9<2539::AID-IMMU2539>3.0.CO;2-9

DO - 10.1002/1521-4141(200109)31:9<2539::AID-IMMU2539>3.0.CO;2-9

M3 - Article

VL - 31

SP - 2539

EP - 2546

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 9

ER -