Abstract
Immature and mature dendritic cells (DC) have been well characterized functionally and phenotypically. Microorganisms or bacterial products such as lipopolysaccharide (LPS) and inflammatory molecules, including tumor necrosis factor (TNF-α), are both believed to activate the DC maturation program which allows DC to initiate and amplify innate and adaptive immune responses. However, there is increasing evidence that the functional state of DC, induced by different stimuli, may be relevant for the immune response outcome. Thus, we compared the transcriptional program of mature, transitional and immature DC, after either LPS or TNF-α stimulation. GeneChip® oligonucleotide microarrays, representing approximately 6,500 murine genes and ESTs, were used for this analysis. A very diverse modulation of gene expression was observed with the two stimuli. Only LPS-treated cells showed a pattern of expression of genes compatible with a definitive growth arrest and with a suitable activation and control of the immune response.
| Original language | English |
|---|---|
| Pages (from-to) | 2539-2546 |
| Number of pages | 8 |
| Journal | European Journal of Immunology |
| Volume | 31 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- Dendritic cell
- Inflammatory stimulus
- Microarray
- Transcription analysis
ASJC Scopus subject areas
- Immunology
Cite this
Transcriptional reprogramming of dendritic cells by differentiation stimuli. / Granucci, Francesca; Vizzardelli, Caterina; Virzi, Ettore; Rescigno, Maria; Ricciardi-Castagnoli, Paola.
In: European Journal of Immunology, Vol. 31, No. 9, 2001, p. 2539-2546.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transcriptional reprogramming of dendritic cells by differentiation stimuli
AU - Granucci, Francesca
AU - Vizzardelli, Caterina
AU - Virzi, Ettore
AU - Rescigno, Maria
AU - Ricciardi-Castagnoli, Paola
PY - 2001
Y1 - 2001
N2 - Immature and mature dendritic cells (DC) have been well characterized functionally and phenotypically. Microorganisms or bacterial products such as lipopolysaccharide (LPS) and inflammatory molecules, including tumor necrosis factor (TNF-α), are both believed to activate the DC maturation program which allows DC to initiate and amplify innate and adaptive immune responses. However, there is increasing evidence that the functional state of DC, induced by different stimuli, may be relevant for the immune response outcome. Thus, we compared the transcriptional program of mature, transitional and immature DC, after either LPS or TNF-α stimulation. GeneChip® oligonucleotide microarrays, representing approximately 6,500 murine genes and ESTs, were used for this analysis. A very diverse modulation of gene expression was observed with the two stimuli. Only LPS-treated cells showed a pattern of expression of genes compatible with a definitive growth arrest and with a suitable activation and control of the immune response.
AB - Immature and mature dendritic cells (DC) have been well characterized functionally and phenotypically. Microorganisms or bacterial products such as lipopolysaccharide (LPS) and inflammatory molecules, including tumor necrosis factor (TNF-α), are both believed to activate the DC maturation program which allows DC to initiate and amplify innate and adaptive immune responses. However, there is increasing evidence that the functional state of DC, induced by different stimuli, may be relevant for the immune response outcome. Thus, we compared the transcriptional program of mature, transitional and immature DC, after either LPS or TNF-α stimulation. GeneChip® oligonucleotide microarrays, representing approximately 6,500 murine genes and ESTs, were used for this analysis. A very diverse modulation of gene expression was observed with the two stimuli. Only LPS-treated cells showed a pattern of expression of genes compatible with a definitive growth arrest and with a suitable activation and control of the immune response.
KW - Dendritic cell
KW - Inflammatory stimulus
KW - Microarray
KW - Transcription analysis
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UR - http://www.scopus.com/inward/citedby.url?scp=0034801738&partnerID=8YFLogxK
U2 - 10.1002/1521-4141(200109)31:9<2539::AID-IMMU2539>3.0.CO;2-9
DO - 10.1002/1521-4141(200109)31:9<2539::AID-IMMU2539>3.0.CO;2-9
M3 - Article
C2 - 11536151
AN - SCOPUS:0034801738
VL - 31
SP - 2539
EP - 2546
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 9
ER -