Transcriptomic analysis of stem cells treated with moringin or cannabidiol: Analogies and differences in inflammation pathways

Luigi Chiricosta, Serena Silvestro, Jacopo Pizzicannella, Francesca Diomede, Placido Bramanti, Oriana Trubiani, Emanuela Mazzon

Research output: Contribution to journalArticlepeer-review

Abstract

Inflammation is a common feature of many neurodegenerative diseases. The treatment of stem cells as a therapeutic approach to repair damage in the central nervous system represents a valid alternative. In this study, using Next-Generation Sequencing (NGS) technology, we analyzed the transcriptomic profile of human Gingival Mesenchymal Stem Cells (hGMSCs) treated with Moringin [4-(α-l-ramanosyloxy)-benzyl isothiocyanate] (hGMSCs-MOR) or with Cannabidiol (hGMSCs-CBD) at dose of 0.5 or 5 µM, respectively. Moreover, we compared their transcriptomic profiles in order to evaluate analogies and differences in pro-and anti-inflammatory pathways. The hGMSCs-MOR selectively downregulate TNF-α signaling from the beginning, reducing the expression of TNF-α receptor while hGMSCs-CBD limit its activity after the process started. The treatment with CBD downregulates the pro-inflammatory pathway mediated by the IL-1 family, including its receptor while MOR is less efficient. Furthermore, both the treatments are efficient in the IL-6 signaling. In particular, CBD reduces the effect of the pro-inflammatory JAK/STAT pathway while MOR enhances the pro-survival PI3K/AKT/mTOR. In addition, both hGMSCs-MOR and hGMSCs-CBD improve the anti-inflammatory activity enhancing the TGF-β pathway.

Original languageEnglish
Article number6039
JournalInternational Journal of Molecular Sciences
Volume20
Issue number23
DOIs
Publication statusPublished - Dec 1 2019

Keywords

  • Cannabidiol
  • Human gingival mesenchymal stem cells
  • Inflammation
  • Moringin
  • Transcriptome analysis

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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