Transcriptomic context of DRD1 is associated with prefrontal activity and behavior during working memory

Leonardo Fazio, Giulio Pergola, Marco Papalino, Pasquale Di Carlo, Anna Monda, Barbara Gelao, Nicola Amoroso, Sabina Tangaro, Antonio Rampino, Teresa Popolizio, Alessandro Bertolino, Giuseppe Blasi

Research output: Contribution to journalArticle

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Abstract

Dopamine D1 receptor (D1R) signaling shapes prefrontal cortex (PFC) activity during working memory (WM). Previous reports found higher WM performance associated with alleles linked to greater expression of the gene coding for D1Rs (DRD1). However, there is no evidence on the relationship between genetic modulation of DRD1 expression in PFC and patterns of prefrontal activity during WM. Furthermore, previous studies have not considered that D1Rs are part of a coregulated molecular environment, which may contribute to D1R-related prefrontal WM processing. Thus, we hypothesized a reciprocal link between a coregulated (i.e., coexpressed) molecular network including DRD1 and PFC activity. To explore this relationship, we used three independent postmortem prefrontal mRNA datasets (total n = 404) to characterize a coexpression network including DRD1. Then, we indexed network coexpression using a measure (polygenic coexpression index—DRD1-PCI) combining the effect of single nucleotide polymorphisms (SNPs) on coexpression. Finally, we associated the DRD1-PCI with WM performance and related brain activity in independent samples of healthy participants (total n = 371). We identified and replicated a coexpression network including DRD1, whose coexpression was correlated with DRD1-PCI. We also found that DRD1-PCI was associated with lower PFC activity and higher WM performance. Behavioral and imaging results were replicated in independent samples. These findings suggest that genetically predicted expression of DRD1 and of its coexpression partners stratifies healthy individuals in terms of WM performance and related prefrontal activity. They also highlight genes and SNPs potentially relevant to pharmacological trials aimed to test cognitive enhancers modulating DRD1 signaling.

Original languageEnglish
Pages (from-to)5582-5587
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number21
DOIs
Publication statusPublished - May 22 2018

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Short-Term Memory
Prefrontal Cortex
Single Nucleotide Polymorphism
Nootropic Agents
Dopamine D1 Receptors
Healthy Volunteers
Alleles
Pharmacology
Gene Expression
Messenger RNA
Brain
Genes

Keywords

  • DRD1
  • FMRI
  • Gene coexpression network
  • Polygenic score
  • Working memory

ASJC Scopus subject areas

  • General

Cite this

Transcriptomic context of DRD1 is associated with prefrontal activity and behavior during working memory. / Fazio, Leonardo; Pergola, Giulio; Papalino, Marco; Carlo, Pasquale Di; Monda, Anna; Gelao, Barbara; Amoroso, Nicola; Tangaro, Sabina; Rampino, Antonio; Popolizio, Teresa; Bertolino, Alessandro; Blasi, Giuseppe.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 21, 22.05.2018, p. 5582-5587.

Research output: Contribution to journalArticle

Fazio, L, Pergola, G, Papalino, M, Carlo, PD, Monda, A, Gelao, B, Amoroso, N, Tangaro, S, Rampino, A, Popolizio, T, Bertolino, A & Blasi, G 2018, 'Transcriptomic context of DRD1 is associated with prefrontal activity and behavior during working memory', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 21, pp. 5582-5587. https://doi.org/10.1073/pnas.1717135115
Fazio, Leonardo ; Pergola, Giulio ; Papalino, Marco ; Carlo, Pasquale Di ; Monda, Anna ; Gelao, Barbara ; Amoroso, Nicola ; Tangaro, Sabina ; Rampino, Antonio ; Popolizio, Teresa ; Bertolino, Alessandro ; Blasi, Giuseppe. / Transcriptomic context of DRD1 is associated with prefrontal activity and behavior during working memory. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 21. pp. 5582-5587.
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AU - Monda, Anna

AU - Gelao, Barbara

AU - Amoroso, Nicola

AU - Tangaro, Sabina

AU - Rampino, Antonio

AU - Popolizio, Teresa

AU - Bertolino, Alessandro

AU - Blasi, Giuseppe

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