Transduction signals induced in rat brain cortex astrocytes by the HIV-1 gp120 glycoprotein

F. Codazzi, G. Racchetti, F. Grohovaz, J. Meldolesi

Research output: Contribution to journalArticle

Abstract

Cultures of rat brain cortex astrocytes were exposed to 10-10-10-9 M of the HIV-1 envelope glycoprotein, gp120. No specific binding was revealed by the iodinated protein, suggesting expression of only a few sites onto the cells. In contrast, two transduction signals were rapidly induced by gp120: increased tyrosine phosphorylation of a ~56 kDa protein and increased [Ca2+](i). This latter effect, present in 1/3 of the investigated astrocytes, consisted in: discrete or biphasic peaks; slowly rising plateaus; and various types of oscillations. Moreover, in apparently unresponsive cells [Ca2+[(i) rose slowly (45 min) to double the resting levels. Rat brain cortex astrocytes thus appear highly sensitive to gp120. The induced array of signals might contribute to neurotoxicity during HIV infection.

Original languageEnglish
Pages (from-to)135-137
Number of pages3
JournalFEBS Letters
Volume384
Issue number2
DOIs
Publication statusPublished - Apr 15 1996

Keywords

  • [Ca](i)
  • astrocyte
  • gp120
  • HIV neurotoxicity
  • tyrosine phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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