TY - CHAP
T1 - Transfection and DNA-mediated gene transfer
AU - Zecchin, Davide
AU - Di Nicolantonio, Federica
PY - 2011
Y1 - 2011
N2 - The advent of recent technologies such as gene expression microarrays and high-throughput sequencing methods has allowed for unveiling the molecular complexity of cancer. However, compared to the genomic discovery stage, the functional characterization of genes that have been found altered (by somatic mutations, rearrangements, or copy number variations) or differentially regulated at the expression level is still lagging behind. In the future, it is anticipated that efforts would be aimed at addressing the impact of such genes on several cancer traits, including tumor formation, dissemination, and response to therapies. These studies would likely have to rely on introducing the gene(s) of interest (in its wild-type or altered version) in cellular models. We describe here a number of techniques to introduce nucleic acids into eukaryotic cells, ranging from conventional plasmid transfection to lentiviral transduction and adeno-associated viral (AAV)-mediated DNA transfer.
AB - The advent of recent technologies such as gene expression microarrays and high-throughput sequencing methods has allowed for unveiling the molecular complexity of cancer. However, compared to the genomic discovery stage, the functional characterization of genes that have been found altered (by somatic mutations, rearrangements, or copy number variations) or differentially regulated at the expression level is still lagging behind. In the future, it is anticipated that efforts would be aimed at addressing the impact of such genes on several cancer traits, including tumor formation, dissemination, and response to therapies. These studies would likely have to rely on introducing the gene(s) of interest (in its wild-type or altered version) in cellular models. We describe here a number of techniques to introduce nucleic acids into eukaryotic cells, ranging from conventional plasmid transfection to lentiviral transduction and adeno-associated viral (AAV)-mediated DNA transfer.
KW - Cancer mutations
KW - Knock-in
KW - Lentiviral vectors
KW - Plasmid
KW - Targeted homologous recombination
KW - Transfection
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U2 - 10.1007/978-1-61779-80-5_35
DO - 10.1007/978-1-61779-80-5_35
M3 - Chapter
AN - SCOPUS:84934443052
SN - 9781617790799
VL - 731
T3 - Methods in Molecular Biology
SP - 435
EP - 450
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -