Transfection and DNA-mediated gene transfer

Davide Zecchin, Federica Di Nicolantonio

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The advent of recent technologies such as gene expression microarrays and high-throughput sequencing methods has allowed for unveiling the molecular complexity of cancer. However, compared to the genomic discovery stage, the functional characterization of genes that have been found altered (by somatic mutations, rearrangements, or copy number variations) or differentially regulated at the expression level is still lagging behind. In the future, it is anticipated that efforts would be aimed at addressing the impact of such genes on several cancer traits, including tumor formation, dissemination, and response to therapies. These studies would likely have to rely on introducing the gene(s) of interest (in its wild-type or altered version) in cellular models. We describe here a number of techniques to introduce nucleic acids into eukaryotic cells, ranging from conventional plasmid transfection to lentiviral transduction and adeno-associated viral (AAV)-mediated DNA transfer.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages435-450
Number of pages16
Volume731
ISBN (Print)9781617790799
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology
Volume731
ISSN (Print)10643745

Keywords

  • Cancer mutations
  • Knock-in
  • Lentiviral vectors
  • Plasmid
  • Targeted homologous recombination
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Zecchin, D., & Di Nicolantonio, F. (2011). Transfection and DNA-mediated gene transfer. In Methods in Molecular Biology (Vol. 731, pp. 435-450). (Methods in Molecular Biology; Vol. 731). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-80-5_35