Transfer of a human chromosomal vector from a hamster cell line to a mouse embryonic stem cell line

Marianna Paulis, Mirella Bensi, Donata Orioli, Chiara Mondello, Giuliano Mazzini, Maurizio D'Incalci, Cristiano Falcioni, Enrico Radaelli, Eugenio Erba, Elena Raimondi, Luigi De Carli

Research output: Contribution to journalArticle

Abstract

Two transchromosomic mouse embryonic stem (ES) sublines (ESMClox1.5 and ESMClox2.1) containing a human minichromosome (MC) were established from a sample of hybrid colonies isolated in fusion experiments between a normal diploid mouse ES line and a Chinese hamster ovary line carrying the MC. DNA cytometric and chromosome analyses of ESMClox1.5 and ESMClox2.1 indicated a mouse chromosome complement with a heteroploid constitution in a subtetraploid range; the karyotypes showed various degrees of polysomy for different chromosomes. A single copy of the MC was found in the majority of cells in all the isolated hybrid colonies and was stably maintained in the established sublines for more than 100 cell generations either with or without the selective agent. No significant differences from the ES parental cells were observed in growth characteristics of the transchromosomic ES sublines. ESMClox1.5 cells were unable to grow in soft agar; when cultured in hanging drops, they formed embryoid bodies, and when inoculated in nude mice, they produced teratomas. They were able to express the early development markers Oct4 and Nanog, as demonstrated by reverse transcription-polymerase chain reaction assay. All these features are in common with the ES parental line. Further research using the transchromosomic ES sublines described here may allow gene expression studies on transferred human minichromosomes and could shed light on the relationships among ploidy, pluripotency, cell transformation, and tumorigenesis.

Original languageEnglish
Pages (from-to)2543-2550
Number of pages8
JournalStem Cells
Volume25
Issue number10
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Cricetinae
Cell Line
Chromosomes
Embryoid Bodies
Ploidies
Constitution and Bylaws
Teratoma
Embryonic Stem Cells
Cricetulus
Diploidy
Karyotype
Nude Mice
Reverse Transcription
Agar
Ovary
Carcinogenesis
Gene Expression
Polymerase Chain Reaction
Mouse Embryonic Stem Cells
DNA

Keywords

  • Cell fusion
  • Human chromosomal vector
  • Mouse embryonic stem cell
  • Ploidy variation
  • Pluripotent gene markers
  • Transchromosomic mouse embryonic stem cell line

ASJC Scopus subject areas

  • Cell Biology

Cite this

Transfer of a human chromosomal vector from a hamster cell line to a mouse embryonic stem cell line. / Paulis, Marianna; Bensi, Mirella; Orioli, Donata; Mondello, Chiara; Mazzini, Giuliano; D'Incalci, Maurizio; Falcioni, Cristiano; Radaelli, Enrico; Erba, Eugenio; Raimondi, Elena; De Carli, Luigi.

In: Stem Cells, Vol. 25, No. 10, 10.2007, p. 2543-2550.

Research output: Contribution to journalArticle

Paulis, M, Bensi, M, Orioli, D, Mondello, C, Mazzini, G, D'Incalci, M, Falcioni, C, Radaelli, E, Erba, E, Raimondi, E & De Carli, L 2007, 'Transfer of a human chromosomal vector from a hamster cell line to a mouse embryonic stem cell line', Stem Cells, vol. 25, no. 10, pp. 2543-2550. https://doi.org/10.1634/stemcells.2007-0052
Paulis, Marianna ; Bensi, Mirella ; Orioli, Donata ; Mondello, Chiara ; Mazzini, Giuliano ; D'Incalci, Maurizio ; Falcioni, Cristiano ; Radaelli, Enrico ; Erba, Eugenio ; Raimondi, Elena ; De Carli, Luigi. / Transfer of a human chromosomal vector from a hamster cell line to a mouse embryonic stem cell line. In: Stem Cells. 2007 ; Vol. 25, No. 10. pp. 2543-2550.
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