Transferability of lipase titrimetric assays: Deductions from an interlaboratory study

P. Arzoglou; C. Goudoula; P. Tsantili; J. M. Lessinger; G. Ferard; V. Mignot; N. Samyn; J. Demeester; A. Lauwers; H. Brettschneider; F. Ceriotti; A. Avranas; C. Profilis; E. Colinet

Transferability of lipase titrimetric assays: Deductions from an interlaboratory study

P. Arzoglou, C. Goudoula, P. Tsantili, J. M. Lessinger, G. Ferard, V. Mignot, N. Samyn, J. Demeester, A. Lauwers, H. Brettschneider, F. Ceriotti, A. Avranas, C. Profilis, E. Colinet

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.

Original language	English
Pages (from-to)	773-777
Number of pages	5
Journal	European Journal of Clinical Chemistry and Clinical Biochemistry
Volume	32
Issue number	10
Publication status	Published - 1994

ASJC Scopus subject areas

Clinical Biochemistry

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Arzoglou, P., Goudoula, C., Tsantili, P., Lessinger, J. M., Ferard, G., Mignot, V., Samyn, N., Demeester, J., Lauwers, A., Brettschneider, H., Ceriotti, F., Avranas, A., Profilis, C., & Colinet, E. (1994). Transferability of lipase titrimetric assays: Deductions from an interlaboratory study. European Journal of Clinical Chemistry and Clinical Biochemistry, 32(10), 773-777.

Transferability of lipase titrimetric assays : Deductions from an interlaboratory study. / Arzoglou, P.; Goudoula, C.; Tsantili, P.; Lessinger, J. M.; Ferard, G.; Mignot, V.; Samyn, N.; Demeester, J.; Lauwers, A.; Brettschneider, H.; Ceriotti, F.; Avranas, A.; Profilis, C.; Colinet, E.

In: European Journal of Clinical Chemistry and Clinical Biochemistry, Vol. 32, No. 10, 1994, p. 773-777.

Research output: Contribution to journal › Article › peer-review

Arzoglou, P, Goudoula, C, Tsantili, P, Lessinger, JM, Ferard, G, Mignot, V, Samyn, N, Demeester, J, Lauwers, A, Brettschneider, H, Ceriotti, F, Avranas, A, Profilis, C & Colinet, E 1994, 'Transferability of lipase titrimetric assays: Deductions from an interlaboratory study', European Journal of Clinical Chemistry and Clinical Biochemistry, vol. 32, no. 10, pp. 773-777.

Arzoglou, P. ; Goudoula, C. ; Tsantili, P. ; Lessinger, J. M. ; Ferard, G. ; Mignot, V. ; Samyn, N. ; Demeester, J. ; Lauwers, A. ; Brettschneider, H. ; Ceriotti, F. ; Avranas, A. ; Profilis, C. ; Colinet, E. / Transferability of lipase titrimetric assays : Deductions from an interlaboratory study. In: European Journal of Clinical Chemistry and Clinical Biochemistry. 1994 ; Vol. 32, No. 10. pp. 773-777.

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abstract = "Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.",

author = "P. Arzoglou and C. Goudoula and P. Tsantili and Lessinger, {J. M.} and G. Ferard and V. Mignot and N. Samyn and J. Demeester and A. Lauwers and H. Brettschneider and F. Ceriotti and A. Avranas and C. Profilis and E. Colinet",

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AU - Lessinger, J. M.

AU - Ferard, G.

AU - Mignot, V.

AU - Samyn, N.

AU - Demeester, J.

AU - Lauwers, A.

AU - Brettschneider, H.

AU - Ceriotti, F.

AU - Avranas, A.

AU - Profilis, C.

AU - Colinet, E.

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AB - Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.

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Transferability of lipase titrimetric assays: Deductions from an interlaboratory study

Abstract

ASJC Scopus subject areas

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