Abstract
Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.
| Original language | English |
|---|---|
| Pages (from-to) | 773-777 |
| Number of pages | 5 |
| Journal | European Journal of Clinical Chemistry and Clinical Biochemistry |
| Volume | 32 |
| Issue number | 10 |
| Publication status | Published - 1994 |
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ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Transferability of lipase titrimetric assays : Deductions from an interlaboratory study. / Arzoglou, P.; Goudoula, C.; Tsantili, P.; Lessinger, J. M.; Ferard, G.; Mignot, V.; Samyn, N.; Demeester, J.; Lauwers, A.; Brettschneider, H.; Ceriotti, F.; Avranas, A.; Profilis, C.; Colinet, E.
In: European Journal of Clinical Chemistry and Clinical Biochemistry, Vol. 32, No. 10, 1994, p. 773-777.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transferability of lipase titrimetric assays
T2 - Deductions from an interlaboratory study
AU - Arzoglou, P.
AU - Goudoula, C.
AU - Tsantili, P.
AU - Lessinger, J. M.
AU - Ferard, G.
AU - Mignot, V.
AU - Samyn, N.
AU - Demeester, J.
AU - Lauwers, A.
AU - Brettschneider, H.
AU - Ceriotti, F.
AU - Avranas, A.
AU - Profilis, C.
AU - Colinet, E.
PY - 1994
Y1 - 1994
N2 - Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.
AB - Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.
UR - http://www.scopus.com/inward/record.url?scp=0028171892&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028171892&partnerID=8YFLogxK
M3 - Article
VL - 32
SP - 773
EP - 777
JO - European Journal of Clinical Chemistry and Clinical Biochemistry
JF - European Journal of Clinical Chemistry and Clinical Biochemistry
SN - 0939-4974
IS - 10
ER -