Transferability of lipase titrimetric assays: Deductions from an interlaboratory study

P. Arzoglou; C. Goudoula; P. Tsantili; J. M. Lessinger; G. Ferard; V. Mignot; N. Samyn; J. Demeester; A. Lauwers; H. Brettschneider; F. Ceriotti; A. Avranas; C. Profilis; E. Colinet

Transferability of lipase titrimetric assays: Deductions from an interlaboratory study

P. Arzoglou, C. Goudoula, P. Tsantili, J. M. Lessinger, G. Ferard, V. Mignot, N. Samyn, J. Demeester, A. Lauwers, H. Brettschneider, F. Ceriotti, A. Avranas, C. Profilis, E. Colinet

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article

Abstract

Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.

Original language	English
Pages (from-to)	773-777
Number of pages	5
Journal	European Journal of Clinical Chemistry and Clinical Biochemistry
Volume	32
Issue number	10
Publication status	Published - 1994

ASJC Scopus subject areas

Clinical Biochemistry

Access to Document

Fingerprint Dive into the research topics of 'Transferability of lipase titrimetric assays: Deductions from an interlaboratory study'. Together they form a unique fingerprint.

Cite this

Arzoglou, P., Goudoula, C., Tsantili, P., Lessinger, J. M., Ferard, G., Mignot, V., Samyn, N., Demeester, J., Lauwers, A., Brettschneider, H., Ceriotti, F., Avranas, A., Profilis, C., & Colinet, E. (1994). Transferability of lipase titrimetric assays: Deductions from an interlaboratory study. European Journal of Clinical Chemistry and Clinical Biochemistry, 32(10), 773-777.

Transferability of lipase titrimetric assays : Deductions from an interlaboratory study. / Arzoglou, P.; Goudoula, C.; Tsantili, P.; Lessinger, J. M.; Ferard, G.; Mignot, V.; Samyn, N.; Demeester, J.; Lauwers, A.; Brettschneider, H.; Ceriotti, F.; Avranas, A.; Profilis, C.; Colinet, E.

In: European Journal of Clinical Chemistry and Clinical Biochemistry, Vol. 32, No. 10, 1994, p. 773-777.

Research output: Contribution to journal › Article

Arzoglou, P, Goudoula, C, Tsantili, P, Lessinger, JM, Ferard, G, Mignot, V, Samyn, N, Demeester, J, Lauwers, A, Brettschneider, H, Ceriotti, F, Avranas, A, Profilis, C & Colinet, E 1994, 'Transferability of lipase titrimetric assays: Deductions from an interlaboratory study', European Journal of Clinical Chemistry and Clinical Biochemistry, vol. 32, no. 10, pp. 773-777.

Arzoglou, P. ; Goudoula, C. ; Tsantili, P. ; Lessinger, J. M. ; Ferard, G. ; Mignot, V. ; Samyn, N. ; Demeester, J. ; Lauwers, A. ; Brettschneider, H. ; Ceriotti, F. ; Avranas, A. ; Profilis, C. ; Colinet, E. / Transferability of lipase titrimetric assays : Deductions from an interlaboratory study. In: European Journal of Clinical Chemistry and Clinical Biochemistry. 1994 ; Vol. 32, No. 10. pp. 773-777.

@article{3b93005438c648df912e7bd8760561d7,

title = "Transferability of lipase titrimetric assays: Deductions from an interlaboratory study",

abstract = "Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.",

author = "P. Arzoglou and C. Goudoula and P. Tsantili and Lessinger, {J. M.} and G. Ferard and V. Mignot and N. Samyn and J. Demeester and A. Lauwers and H. Brettschneider and F. Ceriotti and A. Avranas and C. Profilis and E. Colinet",

year = "1994",

language = "English",

volume = "32",

pages = "773--777",

journal = "European Journal of Clinical Chemistry and Clinical Biochemistry",

issn = "0939-4974",

publisher = "Walter de Gruyter GmbH & Co. KG",

number = "10",

}

TY - JOUR

T1 - Transferability of lipase titrimetric assays

T2 - Deductions from an interlaboratory study

AU - Arzoglou, P.

AU - Goudoula, C.

AU - Tsantili, P.

AU - Lessinger, J. M.

AU - Ferard, G.

AU - Mignot, V.

AU - Samyn, N.

AU - Demeester, J.

AU - Lauwers, A.

AU - Brettschneider, H.

AU - Ceriotti, F.

AU - Avranas, A.

AU - Profilis, C.

AU - Colinet, E.

PY - 1994

Y1 - 1994

N2 - Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.

AB - Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 ± 125 and 2263 ± 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can there fore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.

UR - http://www.scopus.com/inward/record.url?scp=0028171892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028171892&partnerID=8YFLogxK

M3 - Article

C2 - 7865615

VL - 32

SP - 773

EP - 777

JO - European Journal of Clinical Chemistry and Clinical Biochemistry

JF - European Journal of Clinical Chemistry and Clinical Biochemistry

SN - 0939-4974

IS - 10

ER -