Transferrin promotes endothelial cell migration and invasion: implication in cartilage neovascularization

F. Descalzi Cancedda, M. Carlevaro, A. Albini, D. Ribatti, C. Gentili, R. Llenelli, S. Cermelli, R. Cancedda

Research output: Contribution to journalArticle

Abstract

During endochondral bone formation, avascular cartilage differentiates to hypertrophic cartilage that then undergoes erosion and vascularization leading to bone deposition. Resting cartilage produces inhibitors of angiogenesis, shifting to production of angiogenic stimulators in hypertrophic cartilage. A major protein synthesizd by hypertrophic cartilage both in vivo and in vitro is transferrin, ttere we show that transferrin is a major angiogenic molecule released by hypertrophic cartilage, l-;ndoth(,lial ceil migration and invasion is stimulated by transferrins from a number of dif%rent sources, including hypertrophic cartilage. Checkerboard analysis demonstrates that transferrin is a chemotactic and chemokinetic molecule. ('bondrocyte conditioned media show similar properties. Polyclonal antitransferrin antibodies completely block endothelial ('ell migration and invasion induced by purified transferrin and substantially inhibit the activity produced by hypertrophic chondrocytes. Function blocking m. lbs directed against the transferrin receptor similarly reduce the endothelial migratory response. The angiogenic activity of transferrins was confirmed by in vivo assays on chicken egg chorioallantoic membrane, showing promotion of neovascularization by tran. -ferrins purified from ditferent sources including conditioned culture medium.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number9
Publication statusPublished - 1997

Fingerprint

cell invasion
Endothelial cells
Cartilage
transferrin
Transferrin
angiogenesis
cell movement
cartilage
endothelial cells
Cell Movement
Endothelial Cells
Transferrins
Conditioned Culture Medium
Bone
Chorioallantoic Membrane
chorioallantoic membrane
Molecules
Transferrin Receptors
Angiogenesis Inhibitors
chicken eggs

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Descalzi Cancedda, F., Carlevaro, M., Albini, A., Ribatti, D., Gentili, C., Llenelli, R., ... Cancedda, R. (1997). Transferrin promotes endothelial cell migration and invasion: implication in cartilage neovascularization. FASEB Journal, 11(9).

Transferrin promotes endothelial cell migration and invasion : implication in cartilage neovascularization. / Descalzi Cancedda, F.; Carlevaro, M.; Albini, A.; Ribatti, D.; Gentili, C.; Llenelli, R.; Cermelli, S.; Cancedda, R.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Descalzi Cancedda, F, Carlevaro, M, Albini, A, Ribatti, D, Gentili, C, Llenelli, R, Cermelli, S & Cancedda, R 1997, 'Transferrin promotes endothelial cell migration and invasion: implication in cartilage neovascularization', FASEB Journal, vol. 11, no. 9.
Descalzi Cancedda F, Carlevaro M, Albini A, Ribatti D, Gentili C, Llenelli R et al. Transferrin promotes endothelial cell migration and invasion: implication in cartilage neovascularization. FASEB Journal. 1997;11(9).
Descalzi Cancedda, F. ; Carlevaro, M. ; Albini, A. ; Ribatti, D. ; Gentili, C. ; Llenelli, R. ; Cermelli, S. ; Cancedda, R. / Transferrin promotes endothelial cell migration and invasion : implication in cartilage neovascularization. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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AU - Gentili, C.

AU - Llenelli, R.

AU - Cermelli, S.

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