Abstract
During endochondral bone formation, avascular cartilage differentiates to hypertrophic cartilage that then undergoes erosion and vascularization leading to bone deposition. Resting cartilage produces inhibitors of angiogenesis, shifting to production of angiogenic stimulators in hypertrophic cartilage. A major protein synthesizd by hypertrophic cartilage both in vivo and in vitro is transferrin, ttere we show that transferrin is a major angiogenic molecule released by hypertrophic cartilage, l-;ndoth(,lial ceil migration and invasion is stimulated by transferrins from a number of dif%rent sources, including hypertrophic cartilage. Checkerboard analysis demonstrates that transferrin is a chemotactic and chemokinetic molecule. ('bondrocyte conditioned media show similar properties. Polyclonal antitransferrin antibodies completely block endothelial ('ell migration and invasion induced by purified transferrin and substantially inhibit the activity produced by hypertrophic chondrocytes. Function blocking m. lbs directed against the transferrin receptor similarly reduce the endothelial migratory response. The angiogenic activity of transferrins was confirmed by in vivo assays on chicken egg chorioallantoic membrane, showing promotion of neovascularization by tran. -ferrins purified from ditferent sources including conditioned culture medium.
| Original language | English |
|---|---|
| Journal | FASEB Journal |
| Volume | 11 |
| Issue number | 9 |
| Publication status | Published - 1997 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
Transferrin promotes endothelial cell migration and invasion : implication in cartilage neovascularization. / Descalzi Cancedda, F.; Carlevaro, M.; Albini, A.; Ribatti, D.; Gentili, C.; Llenelli, R.; Cermelli, S.; Cancedda, R.
In: FASEB Journal, Vol. 11, No. 9, 1997.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transferrin promotes endothelial cell migration and invasion
T2 - implication in cartilage neovascularization
AU - Descalzi Cancedda, F.
AU - Carlevaro, M.
AU - Albini, A.
AU - Ribatti, D.
AU - Gentili, C.
AU - Llenelli, R.
AU - Cermelli, S.
AU - Cancedda, R.
PY - 1997
Y1 - 1997
N2 - During endochondral bone formation, avascular cartilage differentiates to hypertrophic cartilage that then undergoes erosion and vascularization leading to bone deposition. Resting cartilage produces inhibitors of angiogenesis, shifting to production of angiogenic stimulators in hypertrophic cartilage. A major protein synthesizd by hypertrophic cartilage both in vivo and in vitro is transferrin, ttere we show that transferrin is a major angiogenic molecule released by hypertrophic cartilage, l-;ndoth(,lial ceil migration and invasion is stimulated by transferrins from a number of dif%rent sources, including hypertrophic cartilage. Checkerboard analysis demonstrates that transferrin is a chemotactic and chemokinetic molecule. ('bondrocyte conditioned media show similar properties. Polyclonal antitransferrin antibodies completely block endothelial ('ell migration and invasion induced by purified transferrin and substantially inhibit the activity produced by hypertrophic chondrocytes. Function blocking m. lbs directed against the transferrin receptor similarly reduce the endothelial migratory response. The angiogenic activity of transferrins was confirmed by in vivo assays on chicken egg chorioallantoic membrane, showing promotion of neovascularization by tran. -ferrins purified from ditferent sources including conditioned culture medium.
AB - During endochondral bone formation, avascular cartilage differentiates to hypertrophic cartilage that then undergoes erosion and vascularization leading to bone deposition. Resting cartilage produces inhibitors of angiogenesis, shifting to production of angiogenic stimulators in hypertrophic cartilage. A major protein synthesizd by hypertrophic cartilage both in vivo and in vitro is transferrin, ttere we show that transferrin is a major angiogenic molecule released by hypertrophic cartilage, l-;ndoth(,lial ceil migration and invasion is stimulated by transferrins from a number of dif%rent sources, including hypertrophic cartilage. Checkerboard analysis demonstrates that transferrin is a chemotactic and chemokinetic molecule. ('bondrocyte conditioned media show similar properties. Polyclonal antitransferrin antibodies completely block endothelial ('ell migration and invasion induced by purified transferrin and substantially inhibit the activity produced by hypertrophic chondrocytes. Function blocking m. lbs directed against the transferrin receptor similarly reduce the endothelial migratory response. The angiogenic activity of transferrins was confirmed by in vivo assays on chicken egg chorioallantoic membrane, showing promotion of neovascularization by tran. -ferrins purified from ditferent sources including conditioned culture medium.
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M3 - Article
AN - SCOPUS:33750269320
VL - 11
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 9
ER -