Transformation by basic fibroblast growth factor requires high levels of expression: Comparison with transformation by hst/K-fgf

N. Quarto, D. Talarico, A. Sommer, R. Florkiewicz, C. Basilico, D. B. Rifkin

Research output: Contribution to journalArticle

Abstract

Basic fibroblast growth factor is a potent mitogen for a variety of cell types and has been suggested to have transforming activity. To test this hypothesis, we have introduced a human bFGF cDNA into NIH 3T3 cells either by DNA transfection or by retrovirus infection. We have compared the properties of cell lines obtained with cells prepared similarly but expressing the hst/K-fgf growth factor. While bFGF does not contain an amino terminal signal sequence and is not secreted from cells in which it is synthesized, hst/K-fgf does contain a signal sequence and is secreted from cells. Our results show that the transformed phenotype correlates directly with the level of bFGF expression, since all transformed clones expressed high levels of bFGF, while nontransformed clones expressed comparatively low levels of bFGF. In contrast, even low levels of hst/K-fgf expression resulted in a transformed phenotype. These results suggest that bFGF is an inefficient transforming protein and that this may relate to its lack of secretion.

Original languageEnglish
Pages (from-to)101-110
Number of pages10
JournalOncogene Research
Volume5
Issue number2
Publication statusPublished - 1989

Keywords

  • Basic fibroblast growth factor
  • Cell transformation
  • Kaposi's sarcoma growth factor
  • Tumorigenicity

ASJC Scopus subject areas

  • Cancer Research

Fingerprint Dive into the research topics of 'Transformation by basic fibroblast growth factor requires high levels of expression: Comparison with transformation by hst/K-fgf'. Together they form a unique fingerprint.

  • Cite this