Transformation of rat thyroid epithelial cells by Kirsten murine sarcoma virus

A. Fusco, A. Pinto, F. S. Ambesi-Impiombato, G. Vecchio, N. Tsuchida

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Fischer rat thyroid epithelial cells (FRT) growing continuously in culture were infected with the Kirsten murine sarcoma virus KiMSV(KiMuLV) and found to produce this virus constitutively. Although the morphology of the FRT cells did not change appreciably, the cells became malignant after infection with KiMSV(KiMuLV) as shown by the growth of infected cells in semi-solid media (uninfected FRT cells did not grow) and by the tumorigenicity of infected cells when injected into syngeneic animals (uninfected FRT cells or FRT cells infected with non-transforming retro-viruses were not tumorigenic). The induced tumors morphologically resembled moderately differentiated carcinomas. Two markers of thyroid epithelial differentiation were absent in the original FRT clone and remained unexpressed after transformation. Fully differentiated rat thyroid epithelial cells (FRT-L cells) infected with another strain of the Kirsten murine sarcoma virus, the KiMSV(MolMuLV), were also transformed as demonstrated by the ability also of these cells to grow as carcinomas (after in vitro transformation) in syngeneic animals. Our results clearly demonstrate that the Kirsten murine sarcoma virus can transform in vitro cells of epithelial as well as of fibroblastic origin.

Original languageEnglish
Pages (from-to)655-662
Number of pages8
JournalInternational Journal of Cancer
Volume28
Issue number5
DOIs
Publication statusPublished - 1981

Fingerprint

Kirsten murine sarcoma virus
Viruses
Carcinoma
Thyroid Epithelial Cells
Inbred F344 Rats
Thyroid Gland
Clone Cells
Epithelial Cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Transformation of rat thyroid epithelial cells by Kirsten murine sarcoma virus. / Fusco, A.; Pinto, A.; Ambesi-Impiombato, F. S.; Vecchio, G.; Tsuchida, N.

In: International Journal of Cancer, Vol. 28, No. 5, 1981, p. 655-662.

Research output: Contribution to journalArticle

Fusco, A. ; Pinto, A. ; Ambesi-Impiombato, F. S. ; Vecchio, G. ; Tsuchida, N. / Transformation of rat thyroid epithelial cells by Kirsten murine sarcoma virus. In: International Journal of Cancer. 1981 ; Vol. 28, No. 5. pp. 655-662.
@article{fc80f8797642441e80d401fab020141f,
title = "Transformation of rat thyroid epithelial cells by Kirsten murine sarcoma virus",
abstract = "Fischer rat thyroid epithelial cells (FRT) growing continuously in culture were infected with the Kirsten murine sarcoma virus KiMSV(KiMuLV) and found to produce this virus constitutively. Although the morphology of the FRT cells did not change appreciably, the cells became malignant after infection with KiMSV(KiMuLV) as shown by the growth of infected cells in semi-solid media (uninfected FRT cells did not grow) and by the tumorigenicity of infected cells when injected into syngeneic animals (uninfected FRT cells or FRT cells infected with non-transforming retro-viruses were not tumorigenic). The induced tumors morphologically resembled moderately differentiated carcinomas. Two markers of thyroid epithelial differentiation were absent in the original FRT clone and remained unexpressed after transformation. Fully differentiated rat thyroid epithelial cells (FRT-L cells) infected with another strain of the Kirsten murine sarcoma virus, the KiMSV(MolMuLV), were also transformed as demonstrated by the ability also of these cells to grow as carcinomas (after in vitro transformation) in syngeneic animals. Our results clearly demonstrate that the Kirsten murine sarcoma virus can transform in vitro cells of epithelial as well as of fibroblastic origin.",
author = "A. Fusco and A. Pinto and Ambesi-Impiombato, {F. S.} and G. Vecchio and N. Tsuchida",
year = "1981",
doi = "10.1002/ijc.2910280519",
language = "English",
volume = "28",
pages = "655--662",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - Transformation of rat thyroid epithelial cells by Kirsten murine sarcoma virus

AU - Fusco, A.

AU - Pinto, A.

AU - Ambesi-Impiombato, F. S.

AU - Vecchio, G.

AU - Tsuchida, N.

PY - 1981

Y1 - 1981

N2 - Fischer rat thyroid epithelial cells (FRT) growing continuously in culture were infected with the Kirsten murine sarcoma virus KiMSV(KiMuLV) and found to produce this virus constitutively. Although the morphology of the FRT cells did not change appreciably, the cells became malignant after infection with KiMSV(KiMuLV) as shown by the growth of infected cells in semi-solid media (uninfected FRT cells did not grow) and by the tumorigenicity of infected cells when injected into syngeneic animals (uninfected FRT cells or FRT cells infected with non-transforming retro-viruses were not tumorigenic). The induced tumors morphologically resembled moderately differentiated carcinomas. Two markers of thyroid epithelial differentiation were absent in the original FRT clone and remained unexpressed after transformation. Fully differentiated rat thyroid epithelial cells (FRT-L cells) infected with another strain of the Kirsten murine sarcoma virus, the KiMSV(MolMuLV), were also transformed as demonstrated by the ability also of these cells to grow as carcinomas (after in vitro transformation) in syngeneic animals. Our results clearly demonstrate that the Kirsten murine sarcoma virus can transform in vitro cells of epithelial as well as of fibroblastic origin.

AB - Fischer rat thyroid epithelial cells (FRT) growing continuously in culture were infected with the Kirsten murine sarcoma virus KiMSV(KiMuLV) and found to produce this virus constitutively. Although the morphology of the FRT cells did not change appreciably, the cells became malignant after infection with KiMSV(KiMuLV) as shown by the growth of infected cells in semi-solid media (uninfected FRT cells did not grow) and by the tumorigenicity of infected cells when injected into syngeneic animals (uninfected FRT cells or FRT cells infected with non-transforming retro-viruses were not tumorigenic). The induced tumors morphologically resembled moderately differentiated carcinomas. Two markers of thyroid epithelial differentiation were absent in the original FRT clone and remained unexpressed after transformation. Fully differentiated rat thyroid epithelial cells (FRT-L cells) infected with another strain of the Kirsten murine sarcoma virus, the KiMSV(MolMuLV), were also transformed as demonstrated by the ability also of these cells to grow as carcinomas (after in vitro transformation) in syngeneic animals. Our results clearly demonstrate that the Kirsten murine sarcoma virus can transform in vitro cells of epithelial as well as of fibroblastic origin.

UR - http://www.scopus.com/inward/record.url?scp=0019468850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019468850&partnerID=8YFLogxK

U2 - 10.1002/ijc.2910280519

DO - 10.1002/ijc.2910280519

M3 - Article

VL - 28

SP - 655

EP - 662

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 5

ER -