Transformation-sensitive protein with molecular weight of 45,000 secreted by mouse fibroblasts

G. Tarone, P. Ceschi, M. Prat, P. M. Comoglio

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The [35S]methionine-labeled proteins released in the medium conditioned by normal and transformed mouse fibroblasts have been analyzed by sodium dodecyl sulfate-polyacrylamide electrophoresis. Three major proteins, fibronectin, procollagens, and a protein with a molecular weight of 45,000 (45K protein) have been identified. The 45K protein, which has not yet been described accounts for about 30% of the proteins released by control 3T3 fibroblasts or mouse embryo cultures. Quantitation of the radioactivity incorporated by the 45K protein indicated a 10- to 15-fold decrease in 3T3 fibroblasts transformed by Kirsten, Abelson, or Rous sarcoma viruses. The amounts of fibronectin and procollagens released in the medium by transformed cells were also reduced by factors of 3- and 5-fold, respectively. Pulse chase experiments have shown that the decreased level of the 45K protein in the medium of transformed cells cannot be explained by a reduced rate of secretion or by extracellular proteolytic degradation. It is not known, however, whether decreased synthesis is responsible for this alteration. The 'tumor promoter' phobol myristate acetate, which is known to induce many of the alterations associated with neoplastic cells, also induced 3T3 fibroblasts to release the 45K protein in amounts comparable to that of transformed cells. Thus, this protein represents a new molecular marker of oncoviral transformation.

Original languageEnglish
Pages (from-to)3648-3652
Number of pages5
JournalCancer Research
Volume41
Issue number9 I
Publication statusPublished - 1981

Fingerprint

Fibroblasts
Molecular Weight
Proteins
Procollagen
Fibronectins
Kirsten murine sarcoma virus
Rous sarcoma virus
Myristic Acid
Conditioned Culture Medium
Sodium Dodecyl Sulfate
Methionine
Carcinogens
Radioactivity
Electrophoresis
Acetates
Embryonic Structures

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tarone, G., Ceschi, P., Prat, M., & Comoglio, P. M. (1981). Transformation-sensitive protein with molecular weight of 45,000 secreted by mouse fibroblasts. Cancer Research, 41(9 I), 3648-3652.

Transformation-sensitive protein with molecular weight of 45,000 secreted by mouse fibroblasts. / Tarone, G.; Ceschi, P.; Prat, M.; Comoglio, P. M.

In: Cancer Research, Vol. 41, No. 9 I, 1981, p. 3648-3652.

Research output: Contribution to journalArticle

Tarone, G, Ceschi, P, Prat, M & Comoglio, PM 1981, 'Transformation-sensitive protein with molecular weight of 45,000 secreted by mouse fibroblasts', Cancer Research, vol. 41, no. 9 I, pp. 3648-3652.
Tarone, G. ; Ceschi, P. ; Prat, M. ; Comoglio, P. M. / Transformation-sensitive protein with molecular weight of 45,000 secreted by mouse fibroblasts. In: Cancer Research. 1981 ; Vol. 41, No. 9 I. pp. 3648-3652.
@article{0a0ecb1a4177446eae10468ceae14c43,
title = "Transformation-sensitive protein with molecular weight of 45,000 secreted by mouse fibroblasts",
abstract = "The [35S]methionine-labeled proteins released in the medium conditioned by normal and transformed mouse fibroblasts have been analyzed by sodium dodecyl sulfate-polyacrylamide electrophoresis. Three major proteins, fibronectin, procollagens, and a protein with a molecular weight of 45,000 (45K protein) have been identified. The 45K protein, which has not yet been described accounts for about 30{\%} of the proteins released by control 3T3 fibroblasts or mouse embryo cultures. Quantitation of the radioactivity incorporated by the 45K protein indicated a 10- to 15-fold decrease in 3T3 fibroblasts transformed by Kirsten, Abelson, or Rous sarcoma viruses. The amounts of fibronectin and procollagens released in the medium by transformed cells were also reduced by factors of 3- and 5-fold, respectively. Pulse chase experiments have shown that the decreased level of the 45K protein in the medium of transformed cells cannot be explained by a reduced rate of secretion or by extracellular proteolytic degradation. It is not known, however, whether decreased synthesis is responsible for this alteration. The 'tumor promoter' phobol myristate acetate, which is known to induce many of the alterations associated with neoplastic cells, also induced 3T3 fibroblasts to release the 45K protein in amounts comparable to that of transformed cells. Thus, this protein represents a new molecular marker of oncoviral transformation.",
author = "G. Tarone and P. Ceschi and M. Prat and Comoglio, {P. M.}",
year = "1981",
language = "English",
volume = "41",
pages = "3648--3652",
journal = "Journal of Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "9 I",

}

TY - JOUR

T1 - Transformation-sensitive protein with molecular weight of 45,000 secreted by mouse fibroblasts

AU - Tarone, G.

AU - Ceschi, P.

AU - Prat, M.

AU - Comoglio, P. M.

PY - 1981

Y1 - 1981

N2 - The [35S]methionine-labeled proteins released in the medium conditioned by normal and transformed mouse fibroblasts have been analyzed by sodium dodecyl sulfate-polyacrylamide electrophoresis. Three major proteins, fibronectin, procollagens, and a protein with a molecular weight of 45,000 (45K protein) have been identified. The 45K protein, which has not yet been described accounts for about 30% of the proteins released by control 3T3 fibroblasts or mouse embryo cultures. Quantitation of the radioactivity incorporated by the 45K protein indicated a 10- to 15-fold decrease in 3T3 fibroblasts transformed by Kirsten, Abelson, or Rous sarcoma viruses. The amounts of fibronectin and procollagens released in the medium by transformed cells were also reduced by factors of 3- and 5-fold, respectively. Pulse chase experiments have shown that the decreased level of the 45K protein in the medium of transformed cells cannot be explained by a reduced rate of secretion or by extracellular proteolytic degradation. It is not known, however, whether decreased synthesis is responsible for this alteration. The 'tumor promoter' phobol myristate acetate, which is known to induce many of the alterations associated with neoplastic cells, also induced 3T3 fibroblasts to release the 45K protein in amounts comparable to that of transformed cells. Thus, this protein represents a new molecular marker of oncoviral transformation.

AB - The [35S]methionine-labeled proteins released in the medium conditioned by normal and transformed mouse fibroblasts have been analyzed by sodium dodecyl sulfate-polyacrylamide electrophoresis. Three major proteins, fibronectin, procollagens, and a protein with a molecular weight of 45,000 (45K protein) have been identified. The 45K protein, which has not yet been described accounts for about 30% of the proteins released by control 3T3 fibroblasts or mouse embryo cultures. Quantitation of the radioactivity incorporated by the 45K protein indicated a 10- to 15-fold decrease in 3T3 fibroblasts transformed by Kirsten, Abelson, or Rous sarcoma viruses. The amounts of fibronectin and procollagens released in the medium by transformed cells were also reduced by factors of 3- and 5-fold, respectively. Pulse chase experiments have shown that the decreased level of the 45K protein in the medium of transformed cells cannot be explained by a reduced rate of secretion or by extracellular proteolytic degradation. It is not known, however, whether decreased synthesis is responsible for this alteration. The 'tumor promoter' phobol myristate acetate, which is known to induce many of the alterations associated with neoplastic cells, also induced 3T3 fibroblasts to release the 45K protein in amounts comparable to that of transformed cells. Thus, this protein represents a new molecular marker of oncoviral transformation.

UR - http://www.scopus.com/inward/record.url?scp=0019448189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019448189&partnerID=8YFLogxK

M3 - Article

VL - 41

SP - 3648

EP - 3652

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0008-5472

IS - 9 I

ER -