Transformed human cells release different fibronectin variants than do normal cells

P. Castellani, A. Siri, C. Rosellini, E. Infusini, L. Borsi, L. Zardi

Research output: Contribution to journalArticlepeer-review

Abstract

Fibronectin molecules are dimers composed of subunits whose primary structures may differ. This is due to alternative splicing in at least two regions (ED and IIICS) of the pre-mRNA. Using two monoclonal antibodies specific for two different epitopes of domain 5 (high affinity for heparin), we have quantitatively analyzed the expression of the IIICS sequence in human fibronectins from different sources. The results demonstrated that the percentage of fibronectin subunits containing the IIICS is higher in fibronectins from tumor-derived or simian virus 40-transformed human cells than in fibronectins from human plasma or normal human fibroblasts. Furthermore, we observed that 45-65% of fibronectin subunits from transformed cells or normal embryonic fibroblasts are sialylated on the heparin-binding domain 5, whereas this occurs in only 24-28% of fibronectin subunits from normal adult fibroblasts. On the contrary, no sialylation was observed on domain 5 in fibronectin from human plasma.

Original languageEnglish
Pages (from-to)1671-1677
Number of pages7
JournalJournal of Cell Biology
Volume103
Issue number5
DOIs
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Cell Biology

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