Transformed human cells release different fibronectin variants than do normal cells

P. Castellani, A. Siri, C. Rosellini, E. Infusini, L. Borsi, L. Zardi

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Fibronectin molecules are dimers composed of subunits whose primary structures may differ. This is due to alternative splicing in at least two regions (ED and IIICS) of the pre-mRNA. Using two monoclonal antibodies specific for two different epitopes of domain 5 (high affinity for heparin), we have quantitatively analyzed the expression of the IIICS sequence in human fibronectins from different sources. The results demonstrated that the percentage of fibronectin subunits containing the IIICS is higher in fibronectins from tumor-derived or simian virus 40-transformed human cells than in fibronectins from human plasma or normal human fibroblasts. Furthermore, we observed that 45-65% of fibronectin subunits from transformed cells or normal embryonic fibroblasts are sialylated on the heparin-binding domain 5, whereas this occurs in only 24-28% of fibronectin subunits from normal adult fibroblasts. On the contrary, no sialylation was observed on domain 5 in fibronectin from human plasma.

Original languageEnglish
Pages (from-to)1671-1677
Number of pages7
JournalJournal of Cell Biology
Volume103
Issue number5
DOIs
Publication statusPublished - 1986

Fingerprint

Fibronectins
Fibroblasts
Heparin
Simian virus 40
RNA Precursors
Alternative Splicing
Epitopes
Monoclonal Antibodies
Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Transformed human cells release different fibronectin variants than do normal cells. / Castellani, P.; Siri, A.; Rosellini, C.; Infusini, E.; Borsi, L.; Zardi, L.

In: Journal of Cell Biology, Vol. 103, No. 5, 1986, p. 1671-1677.

Research output: Contribution to journalArticle

Castellani, P. ; Siri, A. ; Rosellini, C. ; Infusini, E. ; Borsi, L. ; Zardi, L. / Transformed human cells release different fibronectin variants than do normal cells. In: Journal of Cell Biology. 1986 ; Vol. 103, No. 5. pp. 1671-1677.
@article{e37588972d8745fba4a75fc516d01fd7,
title = "Transformed human cells release different fibronectin variants than do normal cells",
abstract = "Fibronectin molecules are dimers composed of subunits whose primary structures may differ. This is due to alternative splicing in at least two regions (ED and IIICS) of the pre-mRNA. Using two monoclonal antibodies specific for two different epitopes of domain 5 (high affinity for heparin), we have quantitatively analyzed the expression of the IIICS sequence in human fibronectins from different sources. The results demonstrated that the percentage of fibronectin subunits containing the IIICS is higher in fibronectins from tumor-derived or simian virus 40-transformed human cells than in fibronectins from human plasma or normal human fibroblasts. Furthermore, we observed that 45-65{\%} of fibronectin subunits from transformed cells or normal embryonic fibroblasts are sialylated on the heparin-binding domain 5, whereas this occurs in only 24-28{\%} of fibronectin subunits from normal adult fibroblasts. On the contrary, no sialylation was observed on domain 5 in fibronectin from human plasma.",
author = "P. Castellani and A. Siri and C. Rosellini and E. Infusini and L. Borsi and L. Zardi",
year = "1986",
doi = "10.1083/jcb.103.5.1671",
language = "English",
volume = "103",
pages = "1671--1677",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - Transformed human cells release different fibronectin variants than do normal cells

AU - Castellani, P.

AU - Siri, A.

AU - Rosellini, C.

AU - Infusini, E.

AU - Borsi, L.

AU - Zardi, L.

PY - 1986

Y1 - 1986

N2 - Fibronectin molecules are dimers composed of subunits whose primary structures may differ. This is due to alternative splicing in at least two regions (ED and IIICS) of the pre-mRNA. Using two monoclonal antibodies specific for two different epitopes of domain 5 (high affinity for heparin), we have quantitatively analyzed the expression of the IIICS sequence in human fibronectins from different sources. The results demonstrated that the percentage of fibronectin subunits containing the IIICS is higher in fibronectins from tumor-derived or simian virus 40-transformed human cells than in fibronectins from human plasma or normal human fibroblasts. Furthermore, we observed that 45-65% of fibronectin subunits from transformed cells or normal embryonic fibroblasts are sialylated on the heparin-binding domain 5, whereas this occurs in only 24-28% of fibronectin subunits from normal adult fibroblasts. On the contrary, no sialylation was observed on domain 5 in fibronectin from human plasma.

AB - Fibronectin molecules are dimers composed of subunits whose primary structures may differ. This is due to alternative splicing in at least two regions (ED and IIICS) of the pre-mRNA. Using two monoclonal antibodies specific for two different epitopes of domain 5 (high affinity for heparin), we have quantitatively analyzed the expression of the IIICS sequence in human fibronectins from different sources. The results demonstrated that the percentage of fibronectin subunits containing the IIICS is higher in fibronectins from tumor-derived or simian virus 40-transformed human cells than in fibronectins from human plasma or normal human fibroblasts. Furthermore, we observed that 45-65% of fibronectin subunits from transformed cells or normal embryonic fibroblasts are sialylated on the heparin-binding domain 5, whereas this occurs in only 24-28% of fibronectin subunits from normal adult fibroblasts. On the contrary, no sialylation was observed on domain 5 in fibronectin from human plasma.

UR - http://www.scopus.com/inward/record.url?scp=0023021573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023021573&partnerID=8YFLogxK

U2 - 10.1083/jcb.103.5.1671

DO - 10.1083/jcb.103.5.1671

M3 - Article

C2 - 3023390

AN - SCOPUS:0023021573

VL - 103

SP - 1671

EP - 1677

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 5

ER -