Transforming growth factor-β-induced expression of CD94/NKG2A inhibitory receptors in human T lymphocytes

Stefania Bertone, Francesca Schiavetti, Rosa Bellomo, Chiara Vitale, Marco Ponte, Lorenzo Moretta, Maria C. Mingari

Research output: Contribution to journalArticle

Abstract

Different HLA class I-specific killer inhibitory receptors (KIR) are expressed in vivo by a fraction of activated T cells, predominantly CD8+, in which they may inhibit TCR-mediated cell functions. In an attempt to identify mechanisms leading to KIR expression in T cells, we analyzed the effect of transforming growth factor-β (TGF-β) in T cells responding to bacterial superantigens in vitro. We show that TGF-β induces the expression of CD94/NKG2A in cells responding to toxic shock syndrome toxin 1 or to other staphylococcal superantigens. Remarkably, maximal CD94 expression occurred at (low) TGF-β concentrations which have no substantial effect on lymphocyte proliferation. Maximal CD94 expression occurred when TGF-β was added shortly after the cells were placed in culture. No expression could be induced in CD94/NKG2A-negative T cell clones. Although both CD4+ and CD8+ expressed CD94, the simultaneous expression of NKG2A was mostly confined to CD8+ cells. Monoclonal antibody-mediated cross-linking of CD94/NKG2A led to an impairment of T cell triggering via CD3, as determined in a redirected killing assay using the Fcγ receptor-positive P815 murine target cells.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalEuropean Journal of Immunology
Volume29
Issue number1
DOIs
Publication statusPublished - 1999

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Keywords

  • HLA-class I molecule
  • Inhibitory receptor
  • Superantigen
  • T lymphocyte activation
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Immunology

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