Abstract
Human hepatoma cell lines (Hep 3B-TS, PLC/PRF/5, and Hep G2), sensitive to growth inhibition by transforming growth factor β1 (TGF-β1), express TGF-β receptors type I, type II, and type III. We report that TGF-β1 protein selectively increased steady-state levels of the mRNA for the serine/threonine kinase receptor 1 (SKR1), a member of the TGF-β superfamily receptor genes in these cells, whereas TGF-β1 had little effect on expression of the TGF-β receptor type II gene. This increase of SKR1 mRNA in Hep 3B-TS cells could be detected by Northern blot analysis within 3 h of addition of TGF-β1 to the cells, and enhanced message levels peaked at 12 h as long as TGF-β1 was present in the culture medium. Hep 3B-TR cells which were resistant to TGF-β1 due to lack of both TGF-β receptors type I and type II, expressed SKR1 mRNA, but it was not induced by TGF-β1 protein. The increased expression of SKR1 mRNA in the cells was actinomycin D-sensitive and was not dependent on new protein synthesis. The results indicate that TGF-β1 selectively induces SKR1 message at a transcriptional level by a positive regulator.
| Original language | English |
|---|---|
| Pages (from-to) | 305-313 |
| Number of pages | 9 |
| Journal | Cell Structure and Function |
| Volume | 19 |
| Issue number | 5 |
| Publication status | Published - 1994 |
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Keywords
- Hepatoma cell line
- TGF-β
- TGF-β receptors
ASJC Scopus subject areas
- Cell Biology
- Physiology
- Structural Biology
Cite this
Transforming growth factor β1 selectively increases gene expression of the serine/threonine kinase receptor 1 (SKR1) in human hepatoma cell lines. / Inagaki, M.; Wang, Z.; Carr, B. I.
In: Cell Structure and Function, Vol. 19, No. 5, 1994, p. 305-313.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transforming growth factor β1 selectively increases gene expression of the serine/threonine kinase receptor 1 (SKR1) in human hepatoma cell lines
AU - Inagaki, M.
AU - Wang, Z.
AU - Carr, B. I.
PY - 1994
Y1 - 1994
N2 - Human hepatoma cell lines (Hep 3B-TS, PLC/PRF/5, and Hep G2), sensitive to growth inhibition by transforming growth factor β1 (TGF-β1), express TGF-β receptors type I, type II, and type III. We report that TGF-β1 protein selectively increased steady-state levels of the mRNA for the serine/threonine kinase receptor 1 (SKR1), a member of the TGF-β superfamily receptor genes in these cells, whereas TGF-β1 had little effect on expression of the TGF-β receptor type II gene. This increase of SKR1 mRNA in Hep 3B-TS cells could be detected by Northern blot analysis within 3 h of addition of TGF-β1 to the cells, and enhanced message levels peaked at 12 h as long as TGF-β1 was present in the culture medium. Hep 3B-TR cells which were resistant to TGF-β1 due to lack of both TGF-β receptors type I and type II, expressed SKR1 mRNA, but it was not induced by TGF-β1 protein. The increased expression of SKR1 mRNA in the cells was actinomycin D-sensitive and was not dependent on new protein synthesis. The results indicate that TGF-β1 selectively induces SKR1 message at a transcriptional level by a positive regulator.
AB - Human hepatoma cell lines (Hep 3B-TS, PLC/PRF/5, and Hep G2), sensitive to growth inhibition by transforming growth factor β1 (TGF-β1), express TGF-β receptors type I, type II, and type III. We report that TGF-β1 protein selectively increased steady-state levels of the mRNA for the serine/threonine kinase receptor 1 (SKR1), a member of the TGF-β superfamily receptor genes in these cells, whereas TGF-β1 had little effect on expression of the TGF-β receptor type II gene. This increase of SKR1 mRNA in Hep 3B-TS cells could be detected by Northern blot analysis within 3 h of addition of TGF-β1 to the cells, and enhanced message levels peaked at 12 h as long as TGF-β1 was present in the culture medium. Hep 3B-TR cells which were resistant to TGF-β1 due to lack of both TGF-β receptors type I and type II, expressed SKR1 mRNA, but it was not induced by TGF-β1 protein. The increased expression of SKR1 mRNA in the cells was actinomycin D-sensitive and was not dependent on new protein synthesis. The results indicate that TGF-β1 selectively induces SKR1 message at a transcriptional level by a positive regulator.
KW - Hepatoma cell line
KW - TGF-β
KW - TGF-β receptors
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UR - http://www.scopus.com/inward/citedby.url?scp=0028004350&partnerID=8YFLogxK
M3 - Article
C2 - 7850892
AN - SCOPUS:0028004350
VL - 19
SP - 305
EP - 313
JO - Cell Structure and Function
JF - Cell Structure and Function
SN - 0386-7196
IS - 5
ER -