Transfusion of autologous, alloactivated helper-enriched lymphocytes obtained by cytapheresis to patients with melanoma

G. Sciorelli, F. Ravagnani, C. Gambacorti-Passerini

Research output: Contribution to journalArticle

Abstract

The aim of this study was to standardize the procedures for collecting a large number of lymphocytes from patients with metastatic melanoma, to sort them using a monoclonal antibody expressing 5/9 + helper phenotype, and to stimulate the helper lymphocytes for 48 or 72 hours with a pool of lymphocytes prepared from eight buffy coats of healthy donors. The toxicity of transfusion and the clinical effects are briefly described. A total of four aphereses and infusions (two weekly) were carried out in five patients. The results indicate that the preparation of high numbers of autologous, allostimulated helper lymphocytes is a feasible procedure, and their transfusion after 72 hours of alloactivation is safe for patients.

Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalPlasma Therapy and Transfusion Technology
Volume7
Issue number4
Publication statusPublished - 1986

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Cytapheresis
Melanoma
Lymphocytes
Blood Component Removal
Lymphocyte Count
Monoclonal Antibodies
Tissue Donors
Phenotype

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Transfusion of autologous, alloactivated helper-enriched lymphocytes obtained by cytapheresis to patients with melanoma. / Sciorelli, G.; Ravagnani, F.; Gambacorti-Passerini, C.

In: Plasma Therapy and Transfusion Technology, Vol. 7, No. 4, 1986, p. 469-476.

Research output: Contribution to journalArticle

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