The aim of this study was to standardize the procedures for collecting a large number of lymphocytes from patients with metastatic melanoma, to sort them using a monoclonal antibody expressing 5/9 + helper phenotype, and to stimulate the helper lymphocytes for 48 or 72 hours with a pool of lymphocytes prepared from eight buffy coats of healthy donors. The toxicity of transfusion and the clinical effects are briefly described. A total of four aphereses and infusions (two weekly) were carried out in five patients. The results indicate that the preparation of high numbers of autologous, allostimulated helper lymphocytes is a feasible procedure, and their transfusion after 72 hours of alloactivation is safe for patients.
|Number of pages||8|
|Journal||Plasma Therapy and Transfusion Technology|
|Publication status||Published - 1986|
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