TY - JOUR
T1 - Transgenic mice mimic the methylation pattern of the human HLA-DRα gene
AU - Feriotto, Giordana
AU - Pozzi, Laura
AU - Piva, Roberta
AU - Deledda, Francesco
AU - Barbieri, Rafaella
AU - Nastruzzi, Claudio
AU - Ciucci, Alessandra
AU - Natali, Pier Giorgio
AU - Giacomini, Patrizio
AU - Gambari, Roberto
PY - 1991/3/15
Y1 - 1991/3/15
N2 - The methylation pattern of the human HLA-DRα gene has been studied in different tissues of transgenic mice. Offspring from two transgenic lines was selected for this analysis, carrying the integrated HLA-DRα gene in either single or multiple (8-10) copies per diploid genome. In transgenic animals two distinct methylation patterns of the HLA-DRα gene are generated, due to a complete methylation of all the GCGC and CCGG sites the former, and to unmethylation restricted to one or both the GCGC sites located in the 5′ portion of the HLA-DRα gene, the latter. Unmethylation restricted to the 5′ portion of the HLA-DRα gene is a highly conserved feature in human tissues and in vitro cultured cell lines; therefore, it is concluded that the methylation pattern of the human HLA-DRα transgene may be faithfully reconstituted in transgenic animals. Northern blotting analysis of the RNA isolated from tissues of the transgenic mouse carrying single-copy HLA-DRα transgene demonstrates its tissue specific expression, suggesting that transgenic mice may represent an "in vivo" experimental system to study the relationship between methylation state and transcriptional activation.
AB - The methylation pattern of the human HLA-DRα gene has been studied in different tissues of transgenic mice. Offspring from two transgenic lines was selected for this analysis, carrying the integrated HLA-DRα gene in either single or multiple (8-10) copies per diploid genome. In transgenic animals two distinct methylation patterns of the HLA-DRα gene are generated, due to a complete methylation of all the GCGC and CCGG sites the former, and to unmethylation restricted to one or both the GCGC sites located in the 5′ portion of the HLA-DRα gene, the latter. Unmethylation restricted to the 5′ portion of the HLA-DRα gene is a highly conserved feature in human tissues and in vitro cultured cell lines; therefore, it is concluded that the methylation pattern of the human HLA-DRα transgene may be faithfully reconstituted in transgenic animals. Northern blotting analysis of the RNA isolated from tissues of the transgenic mouse carrying single-copy HLA-DRα transgene demonstrates its tissue specific expression, suggesting that transgenic mice may represent an "in vivo" experimental system to study the relationship between methylation state and transcriptional activation.
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U2 - 10.1016/0006-291X(91)91586-2
DO - 10.1016/0006-291X(91)91586-2
M3 - Article
C2 - 2018494
AN - SCOPUS:0025743314
VL - 175
SP - 459
EP - 466
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -