Transglutaminase 2 in the enterocytes is coeliac specific and gluten dependent

F. Biagi, J. Campanella, U. Laforenza, G. Gastaldi, S. Tritto, M. Grazioli, V. Villanacci, G. R. Corazza

Research output: Contribution to journalArticle

Abstract

Background: Tissue transglutaminase, the coeliac autoantigen, was shown to localise in the enterocytes of coeliac patients and controls. It was speculated that surface tissue transglutaminase has a role in the pathogenesis of coeliac disease. Aims: To study localisation of tissue transglutaminase in different stages of coeliac disease and other enteropathies with and without villous flattening. Methods: Imunofluorescent and immunoblotting assays were used. Duodenal cryostat sections from 23 coeliac patients (10 untreated, 8 treated, 5 potential) and 18 controls (2 autoimmune enteropathy and 16 normal duodenal mucosa) were incubated with an anti-tissue transglutaminase monoclonal antibody. Slides were blindly examined. Results: The immunofluorescent assay showed that monoclonal antibody localised in the subepithelial layer, in the lamina propria, and in the pericryptal connective tissue of all samples. It also bound to surface enterocytes in 8/10 untreated, 1/8 treated, and 3/5 potential coeliac patients. None of the controls showed an epithelial distribution of tissue transglutaminase. Immunoblotting experiments performed in enterocytes freshly isolated from duodenal biopsy confirmed these findings. Conclusion: Epithelial distribution of tissue transglutaminase is specific for coeliac disease rather than due to a non-specific mucosal inflammation. Analysis of different stages of coeliac disease suggests that the epithelial distribution of tissue transglutaminase is gluten dependent.

Original languageEnglish
Pages (from-to)652-658
Number of pages7
JournalDigestive and Liver Disease
Volume38
Issue number9
DOIs
Publication statusPublished - Sep 2006

Fingerprint

Glutens
Enterocytes
Abdomen
Celiac Disease
Epithelium
Immunoblotting
Mucous Membrane
Monoclonal Antibodies
Autoantigens
transglutaminase 2
Connective Tissue
Inflammation
Biopsy

Keywords

  • Coeliac disease
  • Enterocytes
  • Gliadin
  • Tissue transglutaminase

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Transglutaminase 2 in the enterocytes is coeliac specific and gluten dependent. / Biagi, F.; Campanella, J.; Laforenza, U.; Gastaldi, G.; Tritto, S.; Grazioli, M.; Villanacci, V.; Corazza, G. R.

In: Digestive and Liver Disease, Vol. 38, No. 9, 09.2006, p. 652-658.

Research output: Contribution to journalArticle

Biagi, F, Campanella, J, Laforenza, U, Gastaldi, G, Tritto, S, Grazioli, M, Villanacci, V & Corazza, GR 2006, 'Transglutaminase 2 in the enterocytes is coeliac specific and gluten dependent', Digestive and Liver Disease, vol. 38, no. 9, pp. 652-658. https://doi.org/10.1016/j.dld.2006.05.021
Biagi, F. ; Campanella, J. ; Laforenza, U. ; Gastaldi, G. ; Tritto, S. ; Grazioli, M. ; Villanacci, V. ; Corazza, G. R. / Transglutaminase 2 in the enterocytes is coeliac specific and gluten dependent. In: Digestive and Liver Disease. 2006 ; Vol. 38, No. 9. pp. 652-658.
@article{b8e35cdf4e8e44ec97b30c1acba5ae42,
title = "Transglutaminase 2 in the enterocytes is coeliac specific and gluten dependent",
abstract = "Background: Tissue transglutaminase, the coeliac autoantigen, was shown to localise in the enterocytes of coeliac patients and controls. It was speculated that surface tissue transglutaminase has a role in the pathogenesis of coeliac disease. Aims: To study localisation of tissue transglutaminase in different stages of coeliac disease and other enteropathies with and without villous flattening. Methods: Imunofluorescent and immunoblotting assays were used. Duodenal cryostat sections from 23 coeliac patients (10 untreated, 8 treated, 5 potential) and 18 controls (2 autoimmune enteropathy and 16 normal duodenal mucosa) were incubated with an anti-tissue transglutaminase monoclonal antibody. Slides were blindly examined. Results: The immunofluorescent assay showed that monoclonal antibody localised in the subepithelial layer, in the lamina propria, and in the pericryptal connective tissue of all samples. It also bound to surface enterocytes in 8/10 untreated, 1/8 treated, and 3/5 potential coeliac patients. None of the controls showed an epithelial distribution of tissue transglutaminase. Immunoblotting experiments performed in enterocytes freshly isolated from duodenal biopsy confirmed these findings. Conclusion: Epithelial distribution of tissue transglutaminase is specific for coeliac disease rather than due to a non-specific mucosal inflammation. Analysis of different stages of coeliac disease suggests that the epithelial distribution of tissue transglutaminase is gluten dependent.",
keywords = "Coeliac disease, Enterocytes, Gliadin, Tissue transglutaminase",
author = "F. Biagi and J. Campanella and U. Laforenza and G. Gastaldi and S. Tritto and M. Grazioli and V. Villanacci and Corazza, {G. R.}",
year = "2006",
month = "9",
doi = "10.1016/j.dld.2006.05.021",
language = "English",
volume = "38",
pages = "652--658",
journal = "Digestive and Liver Disease",
issn = "1590-8658",
publisher = "Elsevier B.V.",
number = "9",

}

TY - JOUR

T1 - Transglutaminase 2 in the enterocytes is coeliac specific and gluten dependent

AU - Biagi, F.

AU - Campanella, J.

AU - Laforenza, U.

AU - Gastaldi, G.

AU - Tritto, S.

AU - Grazioli, M.

AU - Villanacci, V.

AU - Corazza, G. R.

PY - 2006/9

Y1 - 2006/9

N2 - Background: Tissue transglutaminase, the coeliac autoantigen, was shown to localise in the enterocytes of coeliac patients and controls. It was speculated that surface tissue transglutaminase has a role in the pathogenesis of coeliac disease. Aims: To study localisation of tissue transglutaminase in different stages of coeliac disease and other enteropathies with and without villous flattening. Methods: Imunofluorescent and immunoblotting assays were used. Duodenal cryostat sections from 23 coeliac patients (10 untreated, 8 treated, 5 potential) and 18 controls (2 autoimmune enteropathy and 16 normal duodenal mucosa) were incubated with an anti-tissue transglutaminase monoclonal antibody. Slides were blindly examined. Results: The immunofluorescent assay showed that monoclonal antibody localised in the subepithelial layer, in the lamina propria, and in the pericryptal connective tissue of all samples. It also bound to surface enterocytes in 8/10 untreated, 1/8 treated, and 3/5 potential coeliac patients. None of the controls showed an epithelial distribution of tissue transglutaminase. Immunoblotting experiments performed in enterocytes freshly isolated from duodenal biopsy confirmed these findings. Conclusion: Epithelial distribution of tissue transglutaminase is specific for coeliac disease rather than due to a non-specific mucosal inflammation. Analysis of different stages of coeliac disease suggests that the epithelial distribution of tissue transglutaminase is gluten dependent.

AB - Background: Tissue transglutaminase, the coeliac autoantigen, was shown to localise in the enterocytes of coeliac patients and controls. It was speculated that surface tissue transglutaminase has a role in the pathogenesis of coeliac disease. Aims: To study localisation of tissue transglutaminase in different stages of coeliac disease and other enteropathies with and without villous flattening. Methods: Imunofluorescent and immunoblotting assays were used. Duodenal cryostat sections from 23 coeliac patients (10 untreated, 8 treated, 5 potential) and 18 controls (2 autoimmune enteropathy and 16 normal duodenal mucosa) were incubated with an anti-tissue transglutaminase monoclonal antibody. Slides were blindly examined. Results: The immunofluorescent assay showed that monoclonal antibody localised in the subepithelial layer, in the lamina propria, and in the pericryptal connective tissue of all samples. It also bound to surface enterocytes in 8/10 untreated, 1/8 treated, and 3/5 potential coeliac patients. None of the controls showed an epithelial distribution of tissue transglutaminase. Immunoblotting experiments performed in enterocytes freshly isolated from duodenal biopsy confirmed these findings. Conclusion: Epithelial distribution of tissue transglutaminase is specific for coeliac disease rather than due to a non-specific mucosal inflammation. Analysis of different stages of coeliac disease suggests that the epithelial distribution of tissue transglutaminase is gluten dependent.

KW - Coeliac disease

KW - Enterocytes

KW - Gliadin

KW - Tissue transglutaminase

UR - http://www.scopus.com/inward/record.url?scp=33748505213&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748505213&partnerID=8YFLogxK

U2 - 10.1016/j.dld.2006.05.021

DO - 10.1016/j.dld.2006.05.021

M3 - Article

C2 - 16916632

AN - SCOPUS:33748505213

VL - 38

SP - 652

EP - 658

JO - Digestive and Liver Disease

JF - Digestive and Liver Disease

SN - 1590-8658

IS - 9

ER -