Transglutaminase 5 cross-links human small proline rich prptein 3: Specificity of amino acid residue utilization

L. N. Marekov, A. Paradisi, G. Melino, P. M. Steinert, E. Candi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The complexity of cross-linking of small proline-rich 3 (SPR3) was investigated by a transglutaminase 5 (TG5). The purification of SPR3 was also described. The cross-linking can affect the tryptic peptide in two ways: digestion with trypsin does not occur at cross-linked lysines and cross-linked peptides will have different elution times. It was shown that the utilization of lysines and glutamines by TG5 differs from that observed for TG1 and TG3.

Original languageEnglish
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages351-352
Number of pages2
Publication statusPublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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