Transglutaminase activity is involved in polyamine-induced programmed cell death

Francesco Facchiano, Daniela D'Arcangelo, Antonella Riccomi, Alessandro Lentini, Simone Beninati, Maurizio C. Capogrossi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Natural polyamines, i.e., putrescine, spermidine, and spermine, are ubiquitous molecules essential for cell proliferation and differentiation. In the present study, the effect of polyamines on primary cultures of bovine aortic endothelial cells (BAECs), rat aortic smooth muscle cells (RASMCs), and a human melanoma cell line was examined. While in the absence of fetal calf serum (FCS) polyamines had no effect on viability, in the presence of FCS spermidine and spermine, at concentrations close to physiologic levels, induced a dose-dependent cell death, whereas putrescine was ineffective. RASMCs were significantly more sensitive than other cells. FACS analysis, oligonucleosome ELISA, Hoechst nuclear staining, and Annexin V-FITC quantification showed that cell death was likely due to apoptosis. Cells exposed to spermidine showed a marked increase of intracellular transglutaminase (TGase) activity (∼30-fold over control). Inhibitors of polyamine oxidation or inhibitors of TGase activity prevented polyamine-induced apoptosis. Moreover, tissue TGase overexpression significantly increased cell sensitivity to polyamine, suggesting that this effect is likely related to enhanced intracellular TGase activity. These data indicate that polyamines may modulate cell viability through a novel TGase-dependent process.

Original languageEnglish
Pages (from-to)118-129
Number of pages12
JournalExperimental Cell Research
Volume271
Issue number1
DOIs
Publication statusPublished - Nov 15 2001

Fingerprint

Transglutaminases
Polyamines
Cell Death
Spermidine
Putrescine
Spermine
Smooth Muscle Myocytes
Apoptosis
Fluorescein-5-isothiocyanate
Annexin A5
Serum
Cell Differentiation
Melanoma
Cell Survival
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Cell Proliferation
Staining and Labeling
Cell Line

Keywords

  • Angiogenesis
  • Apoptosis
  • Melanoma cell
  • Polyamine
  • Programmed cell death
  • Transglutaminase
  • Vascular cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Transglutaminase activity is involved in polyamine-induced programmed cell death. / Facchiano, Francesco; D'Arcangelo, Daniela; Riccomi, Antonella; Lentini, Alessandro; Beninati, Simone; Capogrossi, Maurizio C.

In: Experimental Cell Research, Vol. 271, No. 1, 15.11.2001, p. 118-129.

Research output: Contribution to journalArticle

Facchiano, F, D'Arcangelo, D, Riccomi, A, Lentini, A, Beninati, S & Capogrossi, MC 2001, 'Transglutaminase activity is involved in polyamine-induced programmed cell death', Experimental Cell Research, vol. 271, no. 1, pp. 118-129. https://doi.org/10.1006/excr.2001.5356
Facchiano, Francesco ; D'Arcangelo, Daniela ; Riccomi, Antonella ; Lentini, Alessandro ; Beninati, Simone ; Capogrossi, Maurizio C. / Transglutaminase activity is involved in polyamine-induced programmed cell death. In: Experimental Cell Research. 2001 ; Vol. 271, No. 1. pp. 118-129.
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