Transglutaminase participates in the blockade of neurotransmitter release by tetanus toxin

Evidence for a novel biological function

Francesco Facchiano, Florence Deloye, Frédéric Doussau, Giulio Innamorati, Anthony C. Ashton, J. Oliver Dolly, Simone Beninati, Angelo Facchiano, Alberto Luini, Bernard Poulain, Fabio Benfenati

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Inhibition of neuroexocytosis by tetanus neurotoxin (TeNT) involves VAMP-2/synaptobrevin-2 cleavage. However, deletion of the TeNT activity does not completely abolish its inhibitory action. TeNT is a potent activator of the cross-linking enzyme transglutaminase 2 (TGase 2) in vitro. The role of the latter mechanism in TeNT poisoning was investigated in isolated nerve terminals and intact neurons. TeNT-induced inhibition of glutamate release from rat cortical synaptosomes was associated with a simultaneous activation of neuronal transglutaminase (TGase) activity. The TeNT-induced blockade of neuroexocytosis was strongly attenuated by pretreatment of either live Aplysia neurons or isolated nerve terminals with specific TGase inhibitors or neutralizing antibodies. The same treatments completely abolished the residual blockade of neuroexocytosis of a non-proteolytic mutant of TeNT light chain. Electrophysiological studies indicated that TGase activation occurs at an early step of TeNT poisoning and contributes to the inhibition of transmitter release. Bioinformatics and biochemical analyses identified synapsin I and SNAP-25 as potential presynaptic TGase substrates in isolated nerve terminals, which are potentially involved in the inhibitory action of TeNT. The results suggest that neuronal TGase activity plays an important role in the regulation of neuroexocytosis and is one of the intracellular targets of TeNT in neurons.

Original languageEnglish
Pages (from-to)257-269
Number of pages13
JournalAmino Acids
Volume39
Issue number1
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Tetanus Toxin
Transglutaminases
Neurotransmitter Agents
Vesicle-Associated Membrane Protein 2
Neurons
Poisoning
Chemical activation
Synapsins
tetanospasmin
Aplysia
Synaptosomes
Bioinformatics
Neutralizing Antibodies
Computational Biology
Rats
Glutamic Acid
Transmitters

Keywords

  • Melanoma
  • Neuroexocytosis
  • Proteomics
  • SNAP-25
  • Synapsin I
  • Tetanus toxin
  • Transglutaminase 2
  • VAMP/synaptobrevin-2

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Facchiano, F., Deloye, F., Doussau, F., Innamorati, G., Ashton, A. C., Dolly, J. O., ... Benfenati, F. (2010). Transglutaminase participates in the blockade of neurotransmitter release by tetanus toxin: Evidence for a novel biological function. Amino Acids, 39(1), 257-269. https://doi.org/10.1007/s00726-009-0436-3

Transglutaminase participates in the blockade of neurotransmitter release by tetanus toxin : Evidence for a novel biological function. / Facchiano, Francesco; Deloye, Florence; Doussau, Frédéric; Innamorati, Giulio; Ashton, Anthony C.; Dolly, J. Oliver; Beninati, Simone; Facchiano, Angelo; Luini, Alberto; Poulain, Bernard; Benfenati, Fabio.

In: Amino Acids, Vol. 39, No. 1, 06.2010, p. 257-269.

Research output: Contribution to journalArticle

Facchiano, F, Deloye, F, Doussau, F, Innamorati, G, Ashton, AC, Dolly, JO, Beninati, S, Facchiano, A, Luini, A, Poulain, B & Benfenati, F 2010, 'Transglutaminase participates in the blockade of neurotransmitter release by tetanus toxin: Evidence for a novel biological function', Amino Acids, vol. 39, no. 1, pp. 257-269. https://doi.org/10.1007/s00726-009-0436-3
Facchiano, Francesco ; Deloye, Florence ; Doussau, Frédéric ; Innamorati, Giulio ; Ashton, Anthony C. ; Dolly, J. Oliver ; Beninati, Simone ; Facchiano, Angelo ; Luini, Alberto ; Poulain, Bernard ; Benfenati, Fabio. / Transglutaminase participates in the blockade of neurotransmitter release by tetanus toxin : Evidence for a novel biological function. In: Amino Acids. 2010 ; Vol. 39, No. 1. pp. 257-269.
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