Transient exposure to cytarabine increases peptide growth factor receptor expression and tumorigenicity of melanoma cells

Michele Caraglia, Annalisa Leardi, Salvatore Improta, Vilma Perin, Beatrice Ricciardi, Claudio Arra, Paola Ferraro, Antonietta Fabbrocini, Antonio Pinto, A. Raffaele Bianco, Pierosandro Tagliaferri

Research output: Contribution to journalArticlepeer-review

Abstract

We have demonstrated that anticancer drugs at cytostatic concentrations enhance the expression and function of epidermal growth factor (EGF-R) and transferrin (TRF-R) receptors on human tumor cells. We hypothesized that these effects could represent a protective response of tumor cells to sublethal antiproliferative stimuli which could lead to enhanced growth potential. 72 hours exposure of human melanoma GLL-19 cells to 1000 nM ara-C induced growth inhibition and increased the number of EGF-R, TRF-R and nerve growth factor receptor (NGF-R) on cell surface. Enhanced expression of β3 integrins CD49a, CD49c and CD49e, α(v) integrin CD51, β3 integrin CD61, CD58/LFA3 and collagen IV and laminin was also detected in ara-C-treated GLL-19 cells. These changes at the tumor cell surface were paralleled by increased in vitro adhesion, invasive potential and clonogenic growth in soft agar and in vivo tumor formation. A more aggressive tumor cell phenotype is induced in human melanoma cells after transient exposure to cytostatic concentrations of ara-C.

Original languageEnglish
Pages (from-to)2369-2375
Number of pages7
JournalAnticancer Research
Volume17
Issue number4 A
Publication statusPublished - 1997

Keywords

  • Adhesion molecules
  • Cytarabine
  • Melanoma
  • Peptide growth factor receptor
  • Tumorigenicity

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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