Transient focal ischemia triggers neuronal expression of GAT-3 in the rat perilesional cortex

Marcello Melone, Andrea Cozzi, Domenico E. Pellegrini-Giampietro, Fiorenzo Conti

Research output: Contribution to journalArticle

Abstract

We studied the expression of γ-aminobutyric acid plasma membrane transporters GAT-1 and GAT-3 in the cerebral cortex of rats following transient middle cerebral artery occlusion. GAT-1 immunoreactivity exhibited minor changes and immunoblotting studies showed that the protein levels were unchanged. By contrast, GAT-3 immunoreactivity, which is normally expressed exclusively by distal astrocytic processes, was remarkably altered: (1) GAT-3-positive puncta were reduced in the perilesional cortex and disorganized in the remaining regions; (2) numerous GAT-3-positive cells were observed, mostly in the perilesional cortex; they were all NeuN positive and most of them were identified as pyramidal neurons. Immunoblotting studies showed that GAT-3 levels were not significantly altered. Confocal studies showed that virtually all GAT-3-positive neurons in the perilesional cortex were TUNEL negative and coexpressed heat shock protein 70 kDa. These findings suggest that ischemia triggers a novel neuronal expression of GAT-3 that is greatest in the perilesional cortex and may contribute to the reported reduction in GABAergic inhibition.

Original languageEnglish
Pages (from-to)120-132
Number of pages13
JournalNeurobiology of Disease
Volume14
Issue number1
DOIs
Publication statusPublished - Oct 2003

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Ischemia
Immunoblotting
Aminobutyrates
GAT
HSP70 Heat-Shock Proteins
Membrane Transport Proteins
Pyramidal Cells
Middle Cerebral Artery Infarction
In Situ Nick-End Labeling
Cerebral Cortex
Cell Membrane
Neurons
Proteins

ASJC Scopus subject areas

  • Neurology

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Transient focal ischemia triggers neuronal expression of GAT-3 in the rat perilesional cortex. / Melone, Marcello; Cozzi, Andrea; Pellegrini-Giampietro, Domenico E.; Conti, Fiorenzo.

In: Neurobiology of Disease, Vol. 14, No. 1, 10.2003, p. 120-132.

Research output: Contribution to journalArticle

Melone, Marcello ; Cozzi, Andrea ; Pellegrini-Giampietro, Domenico E. ; Conti, Fiorenzo. / Transient focal ischemia triggers neuronal expression of GAT-3 in the rat perilesional cortex. In: Neurobiology of Disease. 2003 ; Vol. 14, No. 1. pp. 120-132.
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